Characterization of macrophages from the bony fish gilthead seabream using an antibody against the macrophage colony-stimulating factor receptor

Developmental and Comparative Immunology
Ivan MuleroVictoriano Mulero

Abstract

Two major professional phagocyte populations have been described in fish, namely granulocytes and monocytes/macrophages. Although the distribution and localization of macrophages have been documented in several teleost species using mainly light and/or electron microscopy, the lack of appropriate markers for these cells has hampered our in-depth knowledge of their biology. We report here the generation of a monospecific rabbit polyclonal antibody against the gilthead seabream macrophage colony-stimulating factor receptor (Mcsfr), which is an excellent marker of macrophages in mammals and the zebrafish. The anti-Mcsfr has been found to be very useful in immunohistochemistry (IHC) to specifically immunostain the purified macrophages (adherent cells) obtained from the head-kidney as well as different cell populations in paraffin-embedded organs, including the head-kidney, spleen, thymus, gills and liver. Unexpectedly, however, no Mcsfr immunoreactive (Mcsfr(+)) cells were observed in the brain and intestine of the gilthead seabream. We also show that the distribution of Mcsfr(+) cells in the head-kidney and the spleen is unaltered following infection with the fish pathogenic bacterium Vibrio anguillarum and that the Il1b-producing...Continue Reading

References

Jan 1, 1993·Developmental and Comparative Immunology·J H RomboutM I Villena
Oct 17, 2001·Developmental and Comparative Immunology·N F NeumannM Belosevic
Nov 3, 2004·Trends in Cell Biology·Fiona J Pixley, E Richard Stanley
Jan 13, 2006·Developmental and Comparative Immunology·Heidi B T HuttenhuisJan H W M Rombout
Nov 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Iván MuleroVictoriano Mulero

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Citations

Jun 12, 2013·Developmental and Comparative Immunology·Barbara A KatzenbackMiodrag Belosevic
May 1, 2009·The Journal of Reproduction and Development·Elena Chaves-PozoAlfonsa García-Ayala
May 6, 2014·Fish & Shellfish Immunology·Carlo C Lazado, Christopher Marlowe A Caipang
Feb 16, 2016·Fish & Shellfish Immunology·Jin-Sol BaeChan-Il Park
Feb 14, 2016·Developmental and Comparative Immunology·Jorge Galindo-VillegasVictoriano Mulero
Dec 3, 2011·Developmental and Comparative Immunology·Irene SalinasJ Oriol Sunyer
Mar 29, 2016·Developmental and Comparative Immunology·Carmen W E Embregts, Maria Forlenza
May 11, 2015·Fish & Shellfish Immunology·Paolo RonzaMaría Isabel Quiroga
Sep 18, 2015·Frontiers in Immunology·Hetron Mweemba Munang'anduØystein Evensen
Dec 24, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Angels RuyraNerea Roher
Mar 17, 2015·Tissue & Cell·Ingvar Leiv Leknes
Jun 13, 2015·Journal of Immunology Research·Hetron Mweemba Munang'andu, Øystein Evensen
Aug 12, 2014·Developmental and Comparative Immunology·N Nuñez OrtizG Scapigliati
Jun 28, 2016·Developmental and Comparative Immunology·Hyung-Jun ParkChan-Il Park
Dec 23, 2015·Biology·María Ángeles EstebanJosé Meseguer
Sep 10, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Víctor H OlavarríaVictoriano Mulero
Dec 11, 2019·Fish & Shellfish Immunology·Qingchao WangZhen Xu

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