PMID: 2107503Feb 1, 1990Paper

Characterization of major surface and excretory-secretory immunogens of Trypanosoma cruzi trypomastigotes and identification of potential protective antigen

Parasitology
M A OuaissiA Capron

Abstract

The surface antigens of Trypanosoma cruzi trypomastigotes were identified by immunoprecipitation and were compared with metabolically labelled excretory-secretory products (ES) released by the parasites in vitro. A series of major immunogenic components in the ES antigens were revealed (160 kDa, 130 kDa and 80-110 kDa). The trypomastigote surface also bears the 130 kDa band and the 80-110 kDa complex. Competition experiments demonstrated the common antigenic structure of the ES and the surface antigens. Two-dimensional analysis of ES antigens immunoprecipitated by human Chagasic serum revealed several spots in the 80-110 kDa region with a wide range of isoelectric points (PI between 5.4 and 6.7). This reflects a charge heterogeneity of these polypeptides. The trypomastigote 85 kDa polypeptide was also identified in the ES antigens by using a monoclonal antibody against this antigen. Two-dimensional analysis of the 85 kDa proteins shed from the surface of trypomastigotes and immunoprecipitated by the monoclonal antibody 155D3 showed 2 major spots: a major part of the 85 kDa polypeptide was found at pH 6.5-6.6, whereas a substantial amount of the antigen was found at pH 5.7. An additional component with molecular weight of approx...Continue Reading

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Citations

Aug 1, 1991·Molecular and Biochemical Parasitology·G D PollevickD O Sánchez
Feb 1, 1997·Acta Tropica·A GruppiE Vottero-Cima
Sep 19, 1998·Immunology·A C Da SilvaP Minoprio
Jan 1, 1995·Parasitology·E E JazinU Pettersson
May 1, 1997·Applied Biochemistry and Biotechnology·Y M Gomes
Nov 26, 2015·The American Journal of Tropical Medicine and Hygiene·Sarah M GunterEric L Brown
Mar 16, 2005·Revista Da Sociedade Brasileira De Medicina Tropical·Adriano Gomes SilvaSilvio Favoreto Junior
Jan 1, 1993·Springer Seminars in Immunopathology·L R Travassos, I C Almeida
May 1, 2018·Frontiers in Immunology·Fabricio MontalvãoCélio Geraldo Freire-de-Lima
Oct 1, 1992·Clinical Microbiology Reviews·H B TanowitzM Wittner

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