Characterization of Malaysian Trichoderma isolates using random amplified microsatellites (RAMS).

Molecular Biology Reports
Shafiquzzaman SiddiqueeMd Mainul Hasan

Abstract

Trichoderma species are commercially applied as biocontrol agents against numerous plant pathogenic fungi due to their production of antifungal metabolites, competition for nutrients and space, and mycoparasitism. However, currently the identification of Trichoderma species from throughout the world based on micro-morphological descriptions is tedious and prone to error. The correct identification of Trichoderma species is important as several traits are species-specific. The Random Amplified Microsatellites (RAMS) analysis done using five primers in this study showed different degrees of the genetic similarity among 42 isolates of this genus. The genetic similarity values were found to be in the range of 12.50-85.11% based on a total of 76 bands scored in the Trichoderma isolates. Of these 76 bands, 96.05% were polymorphic, 3.95% were monomorphic and 16% were exclusive bands. Two bands (250 bp and 200 bp) produced by primer LR-5 and one band (250 bp) by primer P1A were present in all the Trichoderma isolates collected from healthy and infected oil palm plantation soils. Cluster analysis based on UPGMA of the RAMS marker data showed that T. harzianum, T. virens and T. longibrachiatum isolates were grouped into different clades ...Continue Reading

Citations

Dec 30, 2015·World Journal of Microbiology & Biotechnology·Shalini RaiPramod W Ramteke
Feb 14, 2012·Molecular Biology Reports·Farhat Ahmadi AvinVikineswary Sabaratnam
Mar 4, 2014·TheScientificWorldJournal·Farhat Ahmadi AvinSabaratnam Vikineswary

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