Characterization of MB creatine kinase isoform conversion in vitro and in vivo in dogs

The Journal of Clinical Investigation
J J BilladelloD R Abendschein

Abstract

Time-dependent removal of the COOH-terminal lysine residue from each subunit of tissue MM creatine kinase by plasma carboxypeptidase N produces two additional isoforms that are readily separated, thereby permitting sensitive, early detection of acute myocardial infarction. Only two isoforms of MB creatine kinase have been detected in plasma leading to speculation that the COOH-terminal lysine on the B subunit is resistant to hydrolysis. To define the biochemical changes resulting in MB creatine kinase isoform conversion, we incubated highly purified MB creatine kinase from canine myocardium with plasma carboxypeptidase N. Quantitative anion-exchange chromatography of incubation mixtures and serial plasma samples from dogs subjected to coronary occlusion revealed a second, more acidic form evolved with time that was separated from the tissue isoform. Cyanogen bromide digestion of the two isoforms followed by amino acid sequencing of COOH-terminal peptides showed that MB creatine kinase undergoes removal of the COOH-terminal lysine residue from both M and B subunits. An intermediate form lacking lysine on the M subunit was delineated during incubations by the combined use of anion-exchange chromatography and conventional electrop...Continue Reading

References

Mar 15, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·R A WeversJ B Soons
Jul 15, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·R A WeversJ B Soons
Sep 1, 1975·Archives of Biochemistry and Biophysics·G OshimaE G Erdös
Oct 24, 1985·The New England Journal of Medicine·J S IngwallP D Allen
Feb 28, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·M PanteghiniN Pagnoni
Jul 16, 1986·Biochemical and Biophysical Research Communications·J J BilladelloA W Strauss
Feb 1, 1987·Journal of the American College of Cardiology·R Roberts
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D RomanA Strauss
Aug 4, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·A Grace, R Roberts
Nov 1, 1980·Analytical Biochemistry·T H Plummer, M T Kimmel

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Citations

Jan 1, 1993·Veterinary Research Communications·M AktasJ P Braun
Aug 15, 1991·Clinica Chimica Acta; International Journal of Clinical Chemistry·R H ChristensonG S Wagner
Apr 1, 1991·Journal of the American College of Cardiology·P R Puleo, M B Perryman
Aug 1, 1992·Journal of the American College of Cardiology·N A PragerD R Abendschein
Nov 25, 1991·Nucleic Acids Research·M E RitchieJ J Billadello
Jan 1, 1994·Annual Review of Medicine·Y Rozenman, M S Gotsman
Apr 1, 1997·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·M ZaninottoM Plebani

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