Characterization of membrane homing receptors in two cloned murine hemopoietic progenitor cell lines

The Journal of Clinical Investigation
T MatsuokaM Tavassoli

Abstract

To characterize homing receptors that are responsible for the recognition and specific binding of hemopoietic progenitor cells to the stroma, we synthesized and 125I-labeled a number of neoglycoproteins. We used these neoglycoproteins as ligand to detect receptors on the membrane of two cloned murine hemopoietic progenitor cell lines, B6SUT and FDCP-1. Both cell lines demonstrated membrane receptors with galactosyl and mannosyl, but not fucosyl, specificities. B6SUT galactosyl receptors showed a single receptor population with a Kd of about 2.3 X 10(-7) M and 10(6) receptors per cell. Mannosyl receptors demonstrated two components with high and low affinities respectively with Kd of about 2.5 X 10(-8) M and 1.0 X 10(-7) M, and respectively about 7.4 X 10(5) and 3.7 X 10(5) receptors per cell. Comparable data were also obtained for FDCP-1. Displacement experiments indicated that radioactive ligands bound to receptors could be increasingly displaced by homologous cold ligand giving typical sigmoid-shaped curves. Cold mannosyl probe could also displace radioactive galactosyl probe in a similar manner, but cold galactosyl probe displaced radioactive mannosyl ligand with a curve demonstrating two phases, further suggesting two recep...Continue Reading

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Feb 1, 1992·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·L BardosiH J Gabius
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