Characterization of mesenchymal stromal cells derived from full-term umbilical cord blood

Cytotherapy
Maria F MancaCristina Navarrete

Abstract

Multipotent mesenchymal stromal cells (MSC) are of interest for their potential to repair bone and cartilage, and also their immunosuppressive properties. Umbilical cord blood (UCB) is reported to contain MSC, and therefore may be a useful source of these cells for clinical applications. We evaluated protocols for isolating MSC from UCB and characterized the surface phenotype, differentiation potential and immunoregulatory properties of the cells obtained. Ten of 25 UCB units processed yielded MSC-like colonies, with depletion of lineage+ cells providing a higher efficiency. Only two of the cultures could be expanded satisfactorily; the remainder failed to proliferate. One culture generated transformed lines that were grossly aneuploid, had up-regulated TERT transcripts and had lost CD90 expression and the capacity to differentiate. The two propagated UCB-MSC lines were similar to those from bone marrow but were not identical to each other, with differences seen in expression of surface markers and cytoskeletal proteins. Both underwent osteogenesis, but at different rates and to different degrees, while neither generated adipocytes. When added as a third party to a mixed lymphocyte culture, both suppressed proliferation. MSC-li...Continue Reading

References

Aug 24, 1999·Bone Marrow Transplantation·S ArmitageM Contreras
Jun 10, 2000·British Journal of Haematology·A EricesJ J Minguell
May 22, 2002·The Journal of Clinical Investigation·Robert E SchwartzCatherine M Verfaillie
Jun 22, 2002·Nature·Yuehua JiangCatherine M Verfaillie
Jan 9, 2003·Lancet·Christof StammGustav Steinhoff
Mar 11, 2004·The International Journal of Biochemistry & Cell Biology·Frank P Barry, J Mary Murphy
Oct 21, 2004·Blood·Sudeepta Aggarwal, Mark F Pittenger
Dec 8, 2004·Best Practice & Research. Clinical Obstetrics & Gynaecology·Keelin O'Donoghue, Nicholas M Fisk
Apr 23, 2005·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Katarina Le Blanc, Olle Ringdén
Jul 29, 2005·The American Journal of Pathology·James S HagoodMoisés Selman
Sep 2, 2005·British Journal of Cancer·O HopferS Aebi
Oct 21, 2005·Cytotherapy·E M HorwitzUNKNOWN International Society for Cellular Therapy
May 30, 2006·Transplantation·Olle RingdénKatarina Le Blanc
Jun 24, 2006·Journal of Cellular Biochemistry·Reza IzadpanahBruce A Bunnell
Jul 28, 2006·Immunology and Cell Biology·Xi ChenGang Li
Aug 2, 2006·Current Opinion in Immunology·Katarina Le Blanc, Olle Ringdén
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Oct 3, 2006·European Journal of Immunology·Antonio UccelliVito Pistoia
Oct 14, 2006·Stem Cells·Jakub TolarBruce R Blazar

❮ Previous
Next ❯

Citations

Mar 25, 2010·Stem Cells and Development·Simone Maria KluthGesine Kögler
Dec 17, 2009·Tissue Engineering. Part a·Hsing-I HuangShu-Hui Chan
Jan 13, 2012·Rheumatology International·Cristiane Sampaio de MaraI B Coimbra
Sep 30, 2010·Cell Biology International·Xiaochun SunWenrong Xu
Jan 12, 2012·Cell Biology International·Lan-Lan MaXi-Ning Pang
May 3, 2012·Annals of the New York Academy of Sciences·Santiago RouraAntoni Bayes-Genis
Apr 11, 2015·BioMed Research International·Santiago RouraAntoni Bayes-Genis
Jan 19, 2008·Cytotherapy·E M Horwitz
Jul 3, 2015·Stem Cell Research & Therapy·Santiago RouraAntoni Bayes-Genis
May 15, 2013·Hematology/oncology and Stem Cell Therapy·Attiyeh VasaghiArmin Attar
Oct 28, 2014·Frontiers in Neurology·Jingang LiSuzanne L Miller
Jun 15, 2016·British Medical Bulletin·Sadie C SlaterPaolo Madeddu
Apr 5, 2016·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Santiago RouraAntoni Bayes-Genis
Apr 29, 2014·World Journal of Stem Cells·Ana Maria Blanco MartinezFernanda Martins Almeida
Apr 29, 2014·World Journal of Stem Cells·Tokiko Nagamura-Inoue, Haiping He
Jun 17, 2020·Stem Cells Translational Medicine·Adriana BajettoTullio Florio
Oct 29, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Antony J CutlerCristina V Navarrete

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.