Characterization of metabolites in rats after intravenous administration of Salvianolic Acid for Injection by ultra performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry

Biomedical Chromatography : BMC
Jingzhuo MiaoGuoxiang Sun

Abstract

It is an essential requirement to clarify the metabolites of traditional Chinese medicine (TCM) injection that contains numerous ingredients to assess of safe and effective medication in clinic. Salvianolic Acid for Injection (SAFI), made from hydrophilic phenolic acids in Salvia miltiorrhiza Bunge, has been widely used for the treatment of cerebrovascular diseases, but still lacks the information of metabolites in vivo. In the present study, we aimed to holistically characterize the metabolites of main active ingredients in rat plasma, bile, urine and feces following intravenous administration of SAFI. An ultra performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF-MS) method was developed. Combining information of retention behaviors, multistage mass spectra and literature data, a total of 8 prototypes and 52 metabolites were tentatively characterized. Metabolites originated from rosmarinic acid and salvianolic acid B consisted the most amount of identified compounds. Meanwhile, four metabolites derived from salvianolic acid D and five from salvianolic acid B were firstly reported. This study revealed that methylation, sulfation and glucuronidation were the major metabolic pathw...Continue Reading

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