Characterization of microbial mixtures by mass spectrometry

Mass Spectrometry Reviews
Todd R Sandrin, Plamen A Demirev

Abstract

MS applications in microbiology have increased significantly in the past 10 years, due in part to the proliferation of regulator-approved commercial MALDI MS platforms for rapid identification of clinical infections. In parallel, with the expansion of MS technologies in the "omics" fields, novel MS-based research efforts to characterize organismal as well as environmental microbiomes have emerged. Successful characterization of microorganisms found in complex mixtures of other organisms remains a major challenge for researchers and clinicians alike. Here, we review recent MS advances toward addressing that challenge. These include sample preparation methods and protocols, and established, for example, MALDI, as well as newer, for example, atmospheric pressure ionization (API) techniques. MALDI mass spectra of intact cells contain predominantly information on the highly expressed house-keeping proteins used as biomarkers. The API methods are applicable for small biomolecule analysis, for example, phospholipids and lipopeptides, and facilitate species differentiation. MS hardware and techniques, for example, tandem MS, including diverse ion source/mass analyzer combinations are discussed. Relevant examples for microbial mixture c...Continue Reading

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Citations

Oct 26, 2018·Journal of the American Society for Mass Spectrometry·Valentina Z PetukhovaLaura M Sanchez
May 16, 2019·Microorganisms·Ricardo Franco-DuarteCélia F Rodrigues
Sep 6, 2019·Analytical and Bioanalytical Chemistry·Christoph MörtelmaierImke Mulder
Nov 7, 2019·Angewandte Chemie·Brendan M DugganJames J La Clair
Apr 21, 2020·Microbial Biotechnology·Jo De VriezeWilly Verstraete
Feb 25, 2020·Metabolomics : Official Journal of the Metabolomic Society·Tim U H BaumeisterGeorg Pohnert
Oct 4, 2018·Analytical and Bioanalytical Chemistry·Siyu LiuWei Xu
Jan 21, 2021·International Journal of Molecular Sciences·Simone RentschlerHans-Peter Deigner
Oct 14, 2021·Mass Spectrometry Reviews·Natalija Topić PopovićRozelindra Čož-Rakovac

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