Characterization of Micropolyspora faeni antigens.

Infection and Immunity
V P KurupN J Calvanico

Abstract

Culture filtrate antigens of Micropolyspora faeni grown in a synthetic medium in a stirred fermentor were characterized. The culture filtrate antigens were fractionated by preparative isoelectric focusing with a pH gradient of 3.5 to 5.5. The fractions were pooled according to their reaction with rabbit anti-M. faeni sera. A pool containing two major antigens which were resolved by analytical isoelectric focusing and polyacrylamide disc gel electrophoresis was obtained. One antigen was stainable with Coomassie blue and periodic acid-Schiff stain and was determined to have a mass of 51,000 daltons. The other antigen was stainable only with Coomassie blue and was determined to have a mass of 29,000 daltons. When used at 1 mg/ml, this pool reacted with the sera from all patients with farmer's lung disease by immunodiffusion but failed to react with control sera.

References

Feb 1, 1977·Infection and Immunity·V P Kurup, J N Fink
Jan 1, 1979·International Archives of Allergy and Applied Immunology·V P Kurup, J N Fink
Jul 1, 1969·Analytical Biochemistry·R M ZachariusJ J Woodlock
Jan 1, 1974·Annals of the New York Academy of Sciences·R C Roberts
Jan 1, 1973·Scandinavian Journal of Immunology. Supplement·P J Svendsen
Feb 1, 1966·Proceedings of the Society for Experimental Biology and Medicine·D E LaBerge, M A Stahmann
Jun 1, 1963·Proceedings of the Society for Experimental Biology and Medicine·M KOBAYASHIH A DICKIE
Jan 1, 1965·Thorax·J PEPYS, P A JENKINS

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