Characterization of microRNA profiles in the mammary gland tissue of dairy goats at the late lactation, dry period and late gestation stages

PloS One
Rong XuanMing Cheng

Abstract

MicroRNAs (miRNAs) play an important role in regulating mammary gland development and lactation. We previously analyzed miRNA expression profiles in Laoshan dairy goat mammary glands at the early (20 d postpartum), peak (90 d postpartum) and late lactation (210 d postpartum) stages. To further enrich and clarify the miRNA expression profiles during the lactation physiological cycle, we sequenced miRNAs in the mammary gland tissues of Laoshan dairy goats at three newly selected stages: the late lactation (240 d postpartum), dry period (300 d postpartum) and late gestation (140 d after mating) stages. We obtained 4038 miRNAs and 385 important miRNA families, including mir-10, let-7 and mir-9. We also identified 754 differentially expressed miRNAs in the mammary gland tissue at the 3 different stages and 6 groups of miRNA clusters that had unique expression patterns. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that GO terms such as mammary gland development (GO:0030879) and mammary gland morphogenesis (GO:0060443) and important signaling pathways, including the insulin signaling pathway (chx04910), hippo signaling pathway (chx04390) and estrogen signaling pathway (chx04915), were enriched....Continue Reading

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Citations

Feb 11, 2021·Genes·Elitsa IvanovaFabienne Le Provost
Jul 20, 2021·Frontiers in Cell and Developmental Biology·Yue ZhangBinyun Cao
Aug 4, 2021·Reproductive Toxicology·Thalles Fernando Rocha RuizEllen Cristina Rivas Leonel

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Methods Mentioned

BETA
RNA-seq
electrophoresis
PCA

Software Mentioned

miRprimer
R
Bowtie
miRDeep2
FastQC
miRanda
- Blast
DESeq2
Cytoscape
Primer

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