Characterization of mitochondrial prohibitin from Boleophthalmus pectinirostris and evaluation of its possible role in spermatogenesis

Fish Physiology and Biochemistry
Di WangJun-Quan Zhu

Abstract

Prohibitin (PHB) is an evolutionarily conserved mitochondrial membrane protein. It plays a vital role in cell proteolysis, senescence, and apoptosis and is associated with spermatogenesis and sperm quality control in mammals. To study the characteristics of the PHB gene and its potential roles during spermatogenesis in Boleophthalmus pectinirostris, we cloned a 1153-bp full-length cDNA from the testis of B. pectinirostris with an open reading frame of 816 bp, which encodes 272 amino acid residues. Real-time quantitative PCR (qPCR) analysis revealed the presence of phb mRNA in all the tissues examined, with higher expression levels found in the testis, kidney, intestine, and muscle tissues. We examined the localization of phb mRNA during spermatogenesis by in situ hybridization (ISH), showing that phb mRNA was distributed in the periphery of the nucleus in primary and secondary spermatocytes. In spermatid and mature sperm, the phb mRNA gradually moved toward one side, where the flagellum is formed. Immunofluorescence (IF) results showed co-localization of the PHB and mitochondria at different stages during spermatogenesis of B. pectinirostris. The signals obtained for PHB decreased as spermatogenesis proceeded; the strongest det...Continue Reading

References

Nov 15, 1989·Biochemical and Biophysical Research Communications·J K McClungM J Nuell
Apr 27, 2000·Trends in Biochemical Sciences·T Langer
Feb 21, 2002·Cellular and Molecular Life Sciences : CMLS·L G J NijtmansP J Coates
Jun 10, 2003·The Journal of Biological Chemistry·Marta Artal-SanzLeo G J Nijtmans
Nov 5, 2004·Molecular Biology of the Cell·Takashi TatsutaThomas Langer
Apr 13, 2005·Trends in Molecular Medicine·Suresh MishraLiam J Murphy
Apr 28, 2006·The Plant Journal : for Cell and Molecular Biology·Chang Sook AhnHyun-Sook Pai
Jun 27, 2006·Journal of Cellular and Molecular Medicine·Suresh MishraLiam J Murphy
Sep 4, 2007·Trends in Cell Biology·Duncan T BrowmanStephen M Robbins
Feb 1, 1949·The Journal of Urology·O S CULP, J W BEST
Jun 19, 2008·Biochimica Et Biophysica Acta·Carsten Merkwirth, Thomas Langer
Jun 6, 2009·Biochimica Et Biophysica Acta·Sudharsana R AndeSuresh Mishra
Sep 8, 2009·Trends in Endocrinology and Metabolism : TEM·Marta Artal-Sanz, Nektarios Tavernarakis
Nov 6, 2009·Journal of Cell Science·Christof OsmanThomas Langer
Sep 22, 2010·PloS One·Claudia SieversThomas Rudel
Oct 5, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Kwang Suk KoShelly C Lu
Jan 13, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ping ZhouCostantino Iadecola
May 15, 2012·Gene·Yanbin DaiZiping Zhang
Jan 19, 2013·Journal of Receptor and Signal Transduction Research·Tian-Biao Zhou, Yuan-Han Qin
Jul 9, 2013·Molecular and Cellular Endocrinology·Mari S LehtiAnu Sironen
Dec 19, 2013·Journal of Cellular Physiology·Indrajit ChowdhuryKelwyn Thomas
Mar 15, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rong-Yi ChenZongbing You

❮ Previous
Next ❯

Datasets Mentioned

BETA
AAB21614.1

Methods Mentioned

BETA
ubiquitination
PCR
electrophoresis

Software Mentioned

TASSER
BLAST
Primer Premier
MEGA
SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis