Characterization of modification enzyme NukM and engineering of a novel thioether bridge in lantibiotic nukacin ISK-1

Applied Microbiology and Biotechnology
Kouki ShioyaKenji Sonomoto

Abstract

The lantibiotic nukacin ISK-1 is an antimicrobial peptide containing unusual amino acids such as lanthionine and dehydrobutyrine. The nukacin ISK-1 prepeptide (NukA) undergoes posttranslational modifications, such as the dehydration and cyclization reactions required to form the unusual amino acids by the modification enzyme NukM. We have previously constructed a system for the introduction of unusual amino acids into NukA by coexpression of NukM in Escherichia coli. Using this system, we describe the substrate specificity of NukM by the coexpression of a series of NukA mutants. Our results revealed the following characteristics of NukM: (1) its dehydration activity is not coupled to its cyclization activity; (2) its dehydration activity is site-specific; (3) the length of the substrate is important for its dehydration activity. Furthermore, we succeeded in introducing a novel thioether bridge in NukA by replacing an unmodified Ser at position 27 with a Cys residue.

References

Nov 1, 1989·Somatic Cell and Molecular Genetics·A M ChanC S Cooper
Dec 17, 2002·The Journal of General and Applied Microbiology·Ayaaki IshizakiTakayuki Ezaki
Jan 31, 2004·Science·Lili XieWilfred A van der Donk
Feb 11, 2005·Chemical Reviews·Champak ChatterjeeWilfred A van der Donk
Jul 26, 2005·Bioscience, Biotechnology, and Biochemistry·Jun-ichi NagaoKenji Sonomoto
Sep 7, 2005·Biochemical and Biophysical Research Communications·Jun-Ichi NagaoKenji Sonomoto
Nov 3, 2005·Journal of the American Chemical Society·Champak ChatterjeeWilfred A van der Donk
Oct 19, 2006·Journal of Bioscience and Bioengineering·Jun-ichi NagaoKenji Sonomoto
Oct 21, 2006·Chemistry & Biology·Champak ChatterjeeWilfred A van der Donk
Nov 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Amanda L McClerrenWilfred A van der Donk
Sep 11, 2007·Journal of Molecular Microbiology and Biotechnology·Jun-ichi NagaoKenji Sonomoto
Feb 26, 2008·Bioorganic & Medicinal Chemistry Letters·Matthew R Levengood, Wilfred A van der Donk
Jun 24, 2008·Biochemistry·Gregory C PattonWilfred A van der Donk
Jan 1, 1998·Bioscience, Biotechnology, and Biochemistry·H KimuraA Ishizaki

❮ Previous
Next ❯

Citations

Mar 13, 2012·Annual Review of Biochemistry·Patrick J Knerr, Wilfred A van der Donk
Apr 11, 2012·Current Opinion in Biotechnology·Ming-Cheng WuJason Micklefield
Jan 31, 2017·Chemical Reviews·Lindsay M RepkaWilfred A van der Donk
Oct 1, 2011·Organic & Biomolecular Chemistry·Alethea B Tabor

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.