PMID: 69725Aug 1, 1977Paper

Characterization of molecular species carrying gross cell surface antigen

Journal of Virology
H W SnyderE Fleissner

Abstract

The Gross cell surface antigen (GCSA), associated with expression of endogenous Gross-type murine leukemia virus (G-MuLV) in tissues of mice, is defined by the cytotoxic reaction of a C57BL/6 antiserum, anti-AKR spontaneous leukemia K36, with cells of the Gross virus-induced C57BL/6 leukemia, Emale symbolG2. Sequential lactoperoxidase-catalyzed radioiodination of Emale symbolG2 cells, Nonidet P-40 lysis, precipitation with anti-K36 serum, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis identified molecules with properties of polyproteins encoded by the gag region of the viral genome. These cell surface species could also be labeled by in vitro culturing of Emale symbolG2 with radioactive glucosamine. The viral specificity of these molecules and their participation in the GCSA typing system were established as follows. (i) Absorption of anti-K36 serum with GCSA(+), but not GCSA(-), leukemias led to a marked decrease in precipitation of these proteins. (ii) The same Emale symbolG2 cell surface proteins were also precipitated by antisera against the MuLV virion proteins p30 and p15. (iii) Anti-K36 was shown to possess antibodies against Gross virus p30 and p15. (iv) "Clearing" the Emale symbolG2 lysate of molecules r...Continue Reading

References

Jan 1, 1975·The Journal of Experimental Medicine·Y ObataE A Boyse
Oct 15, 1976·International Journal of Cancer. Journal International Du Cancer·N M Hogg
Apr 1, 1977·Journal of Virology·J LedbetterS Emery
Aug 1, 1977·Journal of Virology·J S TungE Fleissner
Jul 1, 1976·The Journal of Experimental Medicine·K KawashimaL J Old
Sep 1, 1976·Journal of Virology·J R StephensonG J Kelloff
Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K KawashimaL J Old
Jan 1, 1975·Cell·R B NasoR B Arlinghaus
Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A L GielkensH Bloemendal
Mar 10, 1975·Biochimica Et Biophysica Acta·R B NasoR B Arlinghaus
May 1, 1976·Journal of Virology·I M KerrD Baltimore
Apr 1, 1974·The Journal of Experimental Medicine·T YoshikiE Fleissner
Sep 15, 1973·International Journal of Cancer. Journal International Du Cancer·J F Ferrer
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J P GrantD P Bolognesi
Nov 1, 1974·Journal of Virology·M FriedmanS G Nathenson
Jul 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·E A BoyseE Stockert
Mar 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·T AokiH A Wood
Feb 22, 1964·Nature·L J OLDE STOCKERT

❮ Previous
Next ❯

Citations

Oct 15, 1979·International Journal of Cancer. Journal International Du Cancer·A K NgR B Herberman
Aug 15, 1978·International Journal of Cancer. Journal International Du Cancer·J Schneider, G Hunsmann
May 17, 1979·Nature·R KurthM Essex
Jan 1, 1981·Immunological Reviews·H Festenstein, W Schmidt
Jul 1, 1986·European Journal of Immunology·A Schäfer, W Schmidt
Feb 1, 1984·Journal of Virology·W Herr
May 1, 1981·Journal of Virology·S ChatterjeeE Hunter
Oct 1, 1979·Journal of Virology·J P BrownR C Nowinski
Jan 1, 1985·Veterinary Immunology and Immunopathology·S E HajosC D Pasqualini
Jun 28, 1982·Biochimica Et Biophysica Acta·R Risser
Aug 1, 1977·Journal of Virology·J S TungE Fleissner
Mar 1, 1980·Journal of Virology·E Buetti, H Diggelmann
Apr 30, 1980·Virology·E Buetti, H Diggelmann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.