PMID: 2503040Aug 18, 1989Paper

Characterization of monoamine oxidase activity present in human granulocytes and lymphocytes

Biochimica Et Biophysica Acta
M D BalsaM Unzeta

Abstract

The characterization of monoamine oxidase (MAO) activity in lymphocytes and granulocytes was studied by using cells prepared from human blood. The specific activities of the enzyme towards beta-phenylethylamine (PEA), benzylamine (Bz), tyramine (TYR) and 5-hydroxytryptamine (5-HT) were found to be 5-times higher in lymphocytes than in granulocytes. The absence of the semicarbazide-sensitive amine oxidase (SSAO) was confirmed by the lack of effect of semicarbazide on the benzylamine oxidation. The presence of MAO-B was corroborated by the inhibition of PEA oxidation with nanomolar deprenyl concentrations and by inhibition of TYR oxidation with high clorgyline concentrations, as well as by the simple sigmoid curve obtained in both cases. These results, together with the substrate preferences, suggest that the MAO activity of human granulocytes and lymphocytes is predominantly of the B form. For each fraction the kinetic constants were determined towards PEA, TYR and Bz as substrates. The Km values were similar for both cellular samples, whereas the Vmax values were higher in lymphocytes than in granulocytes. MAO-B was titrated with [3H]pargyline in order to find out the number of active sites. The corresponding molecular concentr...Continue Reading

References

Nov 19, 1977·Lancet·F OwenG J Riley
Sep 1, 1987·Biochemical Pharmacology·D BalsaM Unzeta
Jul 1, 1968·Biochemical Pharmacology·J P Johnston
Jun 1, 1983·Pharmacological Research Communications·G IgnestiF Buffoni
Jan 1, 1981·Psychopharmacology·M A ReveleyA Coppen
Oct 1, 1980·Journal of Clinical Pathology·V GloverA V Hoffbrand
Jul 1, 1964·Biochemical Pharmacology·S OTSUKA, Y KOBAYASHI

❮ Previous
Next ❯

Citations

Dec 14, 2011·Amino Acids·Franca Marino, Marco Cosentino
Feb 1, 1991·The Journal of Pharmacy and Pharmacology·M D BalsaM Unzeta
Oct 9, 2007·Journal of Neuroimmunology·Jian-Lan JiangJian-Jun Wang
Apr 15, 2006·Cell Biochemistry and Function·Eva Pállinger, G Csaba
Jul 6, 2005·Journal of Neuroimmunology·Yi-Hua QiuYu-Ping Peng
Sep 1, 2015·Frontiers in Pharmacology·Angela Scanzano, Marco Cosentino
Jul 1, 2020·Neural Regeneration Research·Sarah Thomas BroomeAlessandro Castorina
Sep 30, 2019·Developmental and Comparative Immunology·Zhong-Wen Chang, Chin-Chyuan Chang
May 18, 2021·Frontiers in Pharmacology·Mahyar Ostadkarampour, Edward E Putnins

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.