Characterization of monoclonal antibodies against prostate specific antigen produced by genetic immunization

Journal of Immunological Methods
Jari LeinonenUlf-Håkan Stenman

Abstract

Prostate specific antigen (PSA) is the most important marker for prostate cancer. Antibodies against minor variants of PSA may be useful in the development of novel diagnostic tests for prostate cancer, but it has been difficult to produce such antibodies by protein immunization. In this study, we have compared the characteristics of monoclonal antibodies (MAbs) obtained by genetic immunization with those obtained by protein immunization. The whole coding region of PSA-cDNA was cloned in a mammalian expression vector pCDNA-3. Six mice were immunized four times by intra-muscular (i.m.) injection of the PSA-pCDNA3 plasmid. The MAbs produced were characterized with respect to subclass, epitope specificity, binding to various molecular forms of PSA and affinity. After intra-muscular injection of DNA, anti-PSA antibodies were detected in the serum of all mice, but the antibody titers were markedly lower than after protein immunization. After fusion of the spleen cells from the mice, five hybridomas producing MAbs to PSA were obtained. The MAbs were of IgG1 and IgG2a isotype and they all recognized equally different forms of free PSA, namely enzymatically active, nicked and proPSA. Epitope mapping showed that these MAbs reacted with ...Continue Reading

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Citations

Nov 10, 2009·Analytical and Bioanalytical Chemistry·Brian C MacknessMelisenda J McDonald
Apr 25, 2007·Hybridoma·Lorena MaestreGiovanna Roncador
Jul 28, 2016·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·David J HawksworthSharmila Manoj
Apr 7, 2016·Emerging Microbes & Infections·Shuying LiuShan Lu
Apr 18, 2008·Biotechnology Letters·Pieranna Chiarella, Vito Michele Fazio

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