Characterization of monoclonal antibodies against cell wall epitopes of the insect pathogenic fungus, Nomuraea rileyi: differential binding to fungal surfaces and cross-reactivity with host hemocytes and basement membrane components

European Journal of Cell Biology
J C Pendland, D G Boucias

Abstract

Monoclonal antibodies (MAbs) were generated against epitopes on yeast-like hyphal bodies and hyphae of the entomopathogenic hyphomycete, Nomuraea rileyi. Two MAbs (4C10, 2H4) bind to epitopes common to both hyphal bodies and hyphae, whereas MAb 4E9 binds only to hyphal surfaces. 4C10 and 2H4 appear to be directed towards carbohydrate portions of cell surface mannoproteins, as evidenced by similarities in staining patterns between these MAbs and Concanavalin A on Western blots of N. rileyi cell wall extracts. These MAbs cross-react with antigens on blastospore and hyphal surfaces of two other entomopathogenic fungi, Beauveria bassiana and Paecilomyces farinosus in fluorescence microscopy assays, but do not cross-react with a non-entomopathogenic strain of Candida albicans or with Saccharomyces cerevisiae yeasts. MAb 4C10 also cross-reacts with immunocompetent granular hemocytes from Spodoptera exigua (beet armyworm) and Trichoplusia ni (cabbage looper) larvae and with S. exigua plasmatocytes. Electron microscopy revealed that this MAb binds to a component in cytoplasmic granules in the hemocytes, and that surface labeling may be due to the release of this MAb-positive component upon degranulation. MAb 2H4 does not cross-react wi...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1990·Infection and Immunity·J P BoucharaJ M Senet

❮ Previous
Next ❯

Citations

Oct 23, 2012·Die Naturwissenschaften·Thomas ChouvencNan-Yao Su
Sep 3, 2008·Experimental & Applied Acarology·Conny Schütte, Marcel Dicke
Apr 7, 2007·Transgenic Research·Natalie FerryAngharad M R Gatehouse
Apr 15, 2006·Proceedings of the National Academy of Sciences of the United States of America·Chengshu Wang, Raymond J St Leger
Jan 9, 2016·Journal of Invertebrate Pathology·Qiushi WangJan W M van Lent
Jan 3, 2007·The Veterinary Clinics of North America. Exotic Animal Practice·Arnaud Van Wettere, Gregory A Lewbart
May 9, 2006·The Journal of Eukaryotic Microbiology·Verena-Ulrike Bläske-LietzeDrion G Boucias
Aug 15, 2006·The Journal of Animal Ecology·K Mary WebberleyGregory D D Hurst
Nov 22, 2005·Journal of Invertebrate Pathology·Verena-Ulrike Bläske-Lietze, Drion G Boucias
Jan 13, 2006·Journal of Evolutionary Biology·W O H Hughes, J J Boomsma
May 3, 2012·Archives of Insect Biochemistry and Physiology·Qi GaoGraham J Thompson
May 25, 2015·Parasites & Vectors·Emmanuel Dunstand-GuzmánFernando Iván Flores-Pérez
Jun 12, 2016·Developmental and Comparative Immunology·Shelley Anne Adamo
Sep 26, 2006·Advances in Virus Research·Sijun LiuBryony C Bonning
Aug 17, 2002·The Plant Journal : for Cell and Molecular Biology·Astrid T Groot, Marcel Dicke
Jul 18, 2001·The Journal of Eukaryotic Microbiology·D G BouciasM Bott
Jan 5, 2001·The Journal of Eukaryotic Microbiology·J E FuxaE H Weidner
Jul 26, 2005·Journal of Invertebrate Pathology·Louela A CastrilloJohn D Vandenberg
Jun 24, 2017·Archives of Insect Biochemistry and Physiology·Ke ZhongXu-Sheng Liu
Feb 17, 2021·Archives of Insect Biochemistry and Physiology·Li LiXu-Sheng Liu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.