PMID: 9170270May 15, 1997Paper

Characterization of monoclonal antibodies for rapid identification of Actinomyces naeslundii in clinical samples

FEMS Microbiology Letters
T ThurnheerR Gmür

Abstract

The purpose of this study was to generate highly specific serological reagents for the quantitative identification of Actinomyces naeslundii in clinical samples, in particular dental plaque. Balb/c mice were immunized with pasteurized human A. naeslundii strains representing different genospecies and serotypes. Ten hybrid cell lines secreting monoclonal antibodies reactive with A. naeslundii were isolated and characterized. Antibody specificity was determined by indirect immunofluo-rescence and enzyme-linked immunosorbent assay using strains from 59 species and by immunofluorescence analyses of supragingival plaque from 10 gingivitis patients. Nine monoclonal antibodies reacted selectively with A. naeslundii, whereas one additionally bound to Actinomyces israelii. They recognized at least nine different epitopes with characteristic expression patterns among the test strains. Six clusters of antigenically unique or closely related strains could be distinguished. Clusters 1, 4, and 5 represented by 12, 18, and 5 strains, respectively, comprised over 80% of the A. naeslundii strains tested. All reference strains for genospecies 1 grouped with cluster 1. Strains associated with genospecies 2 fell into clusters 4 and 5. Tests with m...Continue Reading

References

Dec 1, 1990·Oral Microbiology and Immunology·G H BowdenS J Challacombe
Nov 1, 1988·Infection and Immunity·J O CisarA L Sandberg
Nov 1, 1983·Infection and Immunity·W E MooreR R Ranney
Nov 1, 1982·Infection and Immunity·W E MooreR R Ranney
Aug 1, 1993·Journal of Dental Research·G BowdenC Schachtele
Jan 1, 1996·Caries Research·P SchüpbachB Guggenheim

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Citations

Aug 17, 2001·Journal of Immunoassay & Immunochemistry·S SingletonR Gmür
Mar 7, 2001·Applied and Environmental Microbiology·M GuggenheimB Guggenheim
Mar 7, 2003·Applied and Environmental Microbiology·Thomas ThurnheerBernhard Guggenheim
Aug 26, 2004·Oral Microbiology and Immunology·J J KammaP C Baehni
Oct 2, 2014·BMC Microbiology·Kai BaoNagihan Bostanci
Feb 22, 2008·Gerodontology·Sergio Sánchez-GarcíaCarmen García-Peña

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