PMID: 7541271Mar 1, 1995Paper

Characterization of monoclonal antibodies to O-antigen of lipopolysaccharide of Actinobacillus pleuropneumoniae serotype 2 and their use in the classification of field isolates

FEMS Immunology and Medical Microbiology
J I Rodríguez-BarbosaE F Rodríguez-Ferri

Abstract

Monoclonal antibodies (mAbs) against Actinobacillus pleuropneumoniae serotype 2 (reference strain Shope 4226 and field isolate F46) were produced. Twelve hybridoma clones were selected against both strains, and all the antibodies secreted were found to be reactive with whole-cell antigen of the homologous strain in ELISA, whereas only one mAb was reactive in slide agglutination test. The predominant antibody classes were IgG2b and IgG3, although IgG1 and IgM were also obtained. Immunoblot assay showed that mAbs could recognize a ladder band profile which is in accordance with the O-antigen of lipopolysaccharide. Most of the epitopes involved were resistant to proteinase K and also to boiling in the presence of sodium dodecyl sulfate and reducing conditions, but they were sensitive to periodic acid. The 12 mAbs recognized neither reference strains of the remaining A. pleuropneumoniae serotypes nor other taxonomically related Gram-negative organisms. The suitability of mAbs for serotyping of field isolates was also examined, and a high correlation (97.4%) was found between the results previously established by indirect hemagglutination with polyclonal rabbit sera and those obtained by ELISA with mAbs. The panel of mAbs described ...Continue Reading

References

Jun 1, 1990·Nihon juigaku zasshi. The Japanese journal of veterinary science·T NakaiK Kume
Apr 8, 1985·Journal of Immunological Methods·M P WoodwardR A Bloodgood
Sep 1, 1989·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·P BeskowB Thafvelin
Dec 1, 1988·Nihon juigaku zasshi. The Japanese journal of veterinary science·S SuzukiM Yamashita
Jan 1, 1986·Methods in Enzymology·N J Hoogenraad, C J Wraight
Dec 27, 1985·Journal of Immunological Methods·S W Pyle, W B Schill
Apr 1, 1993·Comparative Immunology, Microbiology and Infectious Diseases·C B GutiérrezE F Rodríguez Ferri
Jan 1, 1985·Neurochemistry International·D TavellaD M Lam

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