Characterization of monoclonal antibodies to the hemagglutinin-esterase glycoprotein of a bovine coronavirus associated with winter dysentery and cross-reactivity to field isolates.

Journal of Clinical Microbiology
G MilaneS Dea

Abstract

Seven hybridoma cell lines producing monoclonal antibodies (MAbs) to the hemagglutinin-esterase (HE) glycoprotein of bovine coronavirus (BCV) were obtained from BALB/c mice that were immunized with an enriched peplomeric fraction of the winter dysentery (WD)-associated strain BCQ.2590. The specificities of these MAbs to either the dimeric (140-kDa) or the monomeric (65-kDa) form of the HE glycoprotein were determined by Western immunoblotting experiments with purified virus and immunoprecipitation tests with [35S]methionine-labelled infected cell extracts. Four of these anti-HE MAbs inhibited the hemagglutinating activity of the virus and three weakly neutralized its infectivity in vitro. In addition, competition binding assays allowed for the definition of two independent antigenic domains (domains A and D) and two overlapping antigenic domains (domains B and C) for the HE glycoprotein of the WD-associated strain; epitopes located within antigenic domain A were not associated with hemagglutination inhibition (HAI) and virus neutralization activities. In HAI tests, the four anti-HA MAbs defined two distinct antigenic subgroups among 24 BCV field isolates that have been associated with either typical outbreaks of WD or neonatal ...Continue Reading

References

Jul 1, 1992·The Journal of General Virology·J F VautherotJ Laporte
Oct 1, 1991·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·S Dea, S Garzon
Jun 1, 1990·Journal of Clinical Microbiology·D A Benfield, L J Saif
Jan 1, 1989·Archives of Virology·A A el-GhorrI Campbell
Jan 1, 1988·Archives of Virology·S Dea, P Tijssen
Sep 10, 1988·The Veterinary Record·L J SaifR A Heckert
Jul 1, 1985·The Journal of General Virology·C F CrouchL A Babiuk
Dec 1, 1988·The Journal of General Virology·W SpaanM C Horzinek
Nov 1, 1987·The Journal of General Virology·D DeregtL A Babiuk
Feb 1, 1985·Virus Research·B KingD A Brian
May 1, 1982·Journal of Virology·B King, D A Brian

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