Characterization of mouse endoplasmic reticulum methionine-R-sulfoxide reductase

Biochemical and Biophysical Research Communications
Hwa-Young Kim, Vadim N Gladyshev

Abstract

Methionine-R-sulfoxide reductases (MsrBs) catalyze a stereospecific reduction of methionine-R-sulfoxides to methionines in proteins. Mammals possess three MsrB genes. MsrB1 (SelR) is a selenoprotein located in the cytosol and nucleus, MsrB2 (CBS-1) is a mitochondrial protein, and MsrB3 is a recently identified protein with an unusual localization pattern. Human MsrB3 occurs in two protein forms, MsrB3A and MsrB3B, which can be targeted to the endoplasmic reticulum (ER) and mitochondria, respectively. These forms are generated by alternative first exon splicing that introduces contrasting N-terminal signal peptides. Herein, we characterized mouse MsrB3 and found no evidence of alternative splicing of its gene. The ER signal was located upstream of the predicted mitochondrial signal sequence in a single coding region, whose product was targeted to the ER. Although the mitochondrial signal could function if placed at the N-terminus, it did not target MsrB3 to mitochondria as part of the entire coding region. In addition, immunoblot assays detected no mitochondrial MsrB3 in examined mouse tissues. The data suggest that, in mice, MsrB3 is largely or exclusively an ER-resident protein, and that the reduction of methionine-R-sulfoxide...Continue Reading

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Citations

Jul 1, 2007·Biomolecular NMR Assignments·Lena S SalAlexander Dikiy
Jun 23, 2009·The American Journal of Dermatopathology·Wirach M TaungjaruwinaiJens J Thiele
Jun 19, 2008·Biological Reviews of the Cambridge Philosophical Society·Xing-Hai Zhang, Herbert Weissbach
Mar 18, 2006·BMC Molecular Biology·Hwa-Young Kim, Vadim N Gladyshev
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Nov 17, 2011·Biochemical and Biophysical Research Communications·Seung Hee Choi, Hwa-Young Kim
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