Characterization of mouse fetal lung cells cultured on a pigskin substrate

In Vitro
Y YoshidaA E Freeman

Abstract

Lung organ bits taken from full-term mice were explanted on the dermal surface of sterile, dead pigskin. The cells migrated onto the pigskin dermis and proliferated to form an organoid culture consisting of ductular structures separated by a matrix of epithelial cells. Cells within the ductular structures were ciliated, produced mucin, and exhibited the activities of nonspecific esterase and gamma-glutamyl transferase; therefore they were considered to be derived from bronchial epithelium. Cells forming the matrix possessed the activities of nonspecific esterase and alkaline phosphatase and contained lamellar structures typical of surfactant-producing pneumocyte Type II cells; therefore they were considered to be derived from alveolar precursor cells.

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Citations

Mar 1, 1996·In Vitro Cellular & Developmental Biology. Animal·J M Cook-MillsT L Feldbush
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A E FreemanE Ruoslahti
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A E Freeman, R M Hoffman
Oct 1, 1987·Analytical Biochemistry·H K KleinmanG C Sephel
Oct 1, 1993·Critical Reviews in Oncology/hematology·R M Hoffman

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