Abstract
The unit describes functional assays for the quantification of mouse hematopoietic stem cells and progenitor cells. The competitive repopulating unit (CRU) assay detects transplantable mouse hematopoietic stem cells with the capacity to regenerate all of the blood cell lineages for extended time periods in vivo. The long-term culture-initiating cell (LTC-IC) assay, founded on the bone marrow long-term culture system, measures primitive hematopoietic progenitors based on their capacity to produce myeloid progeny for at least four weeks. Colony-forming cell (CFC) assays, performed in semisolid medium cultures to assess mouse pre-B, megakaryocyte, erythroid, granulocyte-monocyte, and multipotential hematopoietic progenitors are also described. These assays are powerful tools for evaluating human stem cell (HSC) and progenitor content in various hematopoietic tissues, during development as well as in the adult animal, and in cell populations manipulated ex vivo.
References
Jun 1, 1977·Journal of Cellular Physiology·T M DexterL G Lajtha
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S J SzilvassyC J Eaves
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H J SutherlandC J Eaves
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S J SzilvassyR K Humphries
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C A Whitlock, O N Witte
Mar 12, 1982·Journal of Immunological Methods· Fazekas de St Groth
Jan 1, 1995·The Journal of Experimental Medicine·M Trevisan, N N Iscove
Jul 12, 1996·Science·M OsawaH Nakauchi
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·C L Miller, C J Eaves
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·J AudetC J Eaves
May 27, 2003·Nature Immunology·Patricia BenvenisteNorman N Iscove
Dec 3, 2003·Blood·Daniel J PearceDominique Bonnet
Dec 10, 2003·Experimental Hematology·Naoyuki UchidaConnie J Eaves
Jan 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·David BryderSten-Eirik W Jacobsen
Jan 28, 2004·The Journal of Experimental Medicine·Hina TakanoHiromitsu Nakauchi
Aug 1, 2006·The American Journal of Pathology·David BryderIrving L Weissman
Oct 4, 2006·The Journal of Clinical Investigation·Michelle B BowieConnie J Eaves
Sep 5, 2008·Current Protocols in Cytometry·C C Stewart, S J Stewart
Citations
Dec 25, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Aziza P ManceurJulie Audet
Oct 19, 2010·Genes & Development·María García-FernándezHermann Steller
Dec 5, 2016·Experimental Hematology·Sakiko SuzukiGlen D Raffel
Jun 29, 2017·ELife·Elodie LucheBeatrice Cousin
Jan 13, 2018·Cell Death and Differentiation·Lauriane CabonSantos A Susin
Jul 14, 2016·F1000Research·Hector Mayani
Sep 17, 2010·Stem Cells·Sandrine PoglioBéatrice Cousin
Jan 24, 2012·The EMBO Journal·Melissa A FischerScott W Hiebert
Apr 4, 2012·Radiation Research·Lynnette H CaryMark H Whitnall
Mar 13, 2015·Blood·Connie J Eaves
Apr 17, 2019·BMC Biotechnology·Steven MayersJulie Audet
Jan 11, 2017·Science Advances·Ji Sun Choi, Brendan A C Harley
Sep 14, 2018·Science Translational Medicine·Yun-Ruei KaoBritta Will
Dec 22, 2009·The Analyst·Kerol BartolovicSteven J Howe
Mar 13, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Vahe AkbarianJulie Audet
May 7, 2013·Nature Medicine·Daniel LucasPaul S Frenette
May 3, 2014·Bio-protocol·Marc A Kerenyi
Aug 18, 2018·Scientific Reports·Karen LakyPamela A Frischmeyer-Guerrerio
Sep 23, 2020·Nature Cell Biology·Longyong XuXi Chen
Apr 29, 2015·The EMBO Journal·Min Hee ParkJae-sung Bae
Aug 5, 2021·Npj Regenerative Medicine·Lise RabillerBéatrice Cousin
Apr 20, 2016·Stem Cells·Min Hee ParkJae-Sung Bae
Dec 27, 2011·Experimental Hematology·Maura GasparettoClay Smith