Characterization of mouse hematopoietic stem and progenitor cells

Current Protocols in Immunology
Cindy L MillerC J Eaves

Abstract

The unit describes functional assays for the quantification of mouse hematopoietic stem cells and progenitor cells. The competitive repopulating unit (CRU) assay detects transplantable mouse hematopoietic stem cells with the capacity to regenerate all of the blood cell lineages for extended time periods in vivo. The long-term culture-initiating cell (LTC-IC) assay, founded on the bone marrow long-term culture system, measures primitive hematopoietic progenitors based on their capacity to produce myeloid progeny for at least four weeks. Colony-forming cell (CFC) assays, performed in semisolid medium cultures to assess mouse pre-B, megakaryocyte, erythroid, granulocyte-monocyte, and multipotential hematopoietic progenitors are also described. These assays are powerful tools for evaluating human stem cell (HSC) and progenitor content in various hematopoietic tissues, during development as well as in the adult animal, and in cell populations manipulated ex vivo.

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Citations

Dec 25, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Aziza P ManceurJulie Audet
Oct 19, 2010·Genes & Development·María García-FernándezHermann Steller
Dec 5, 2016·Experimental Hematology·Sakiko SuzukiGlen D Raffel
Jul 14, 2016·F1000Research·Hector Mayani
Sep 17, 2010·Stem Cells·Sandrine PoglioBéatrice Cousin
Apr 4, 2012·Radiation Research·Lynnette H CaryMark H Whitnall
Sep 14, 2018·Science Translational Medicine·Yun-Ruei KaoBritta Will
Mar 13, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Vahe AkbarianJulie Audet
May 7, 2013·Nature Medicine·Daniel LucasPaul S Frenette
May 3, 2014·Bio-protocol·Marc A Kerenyi
Dec 27, 2011·Experimental Hematology·Maura GasparettoClay Smith

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