Characterization of mouse MAGE-derived H-2Kb-restricted CTL epitopes

European Journal of Immunology
Andreas O EggertJürgen C Becker

Abstract

Immunogenic peptide epitopes from tumor-associated antigens serve as targets for cellular immune responses in numerous clinical trials for therapeutic vaccinations. From these it became evident that prevailing questions can only be addressed in animal models. Hence, problems arise from the fact that while for human melanoma many different immunogenic peptide epitopes are known, for mouse melanoma the available selection is very restricted. To overcome this limitation, we applied reverse immunology to identify Kb-restricted epitopes derived of mouse MAGE. Two epitopes which bind strongly to Kb were selected to test for their immunogenicity in vivo. Spleen cells from mice vaccinated by intradermal injection of mature dendritic cells pulsed with these peptides displayed reactivity to the respective epitopes as measured by enzyme-linked immunospot assays and tetramer staining. The processing and presentation of these epitopes was evident by the killing of melanoma cells by the vaccination-induced T cells. Moreover, intravenous challenge with syngeneic melanoma cells demonstrated the protective immunity induced by this vaccination. In summary, we demonstrate the immunogenicity of two Kb-restricted peptide epitopes derived from mouse...Continue Reading

References

Nov 3, 1995·International Journal of Cancer. Journal International Du Cancer·F BrasseurY Humblet
Feb 3, 1997·The Journal of Experimental Medicine·M B BloomJ C Yang
Nov 22, 1997·Current Opinion in Immunology·B J Van den Eynde, P van der Bruggen
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·W W OverwijkN P Restifo
Sep 17, 1999·Tissue Antigens·M H AndersenJ S Haurum
Dec 22, 1999·Immunogenetics·H RammenseeS Stevanović
Dec 18, 2001·International Journal of Cancer. Journal International Du Cancer·M H AndersenE Kämpgen
Apr 17, 2002·Expert Opinion on Biological Therapy·Gopi Shankar, Patricia A Lodge
Jan 9, 2003·Expert Opinion on Biological Therapy·Michael A MorseH Kim Lyerly
May 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·David SchramaJürgen C Becker
Jun 16, 2004·International Journal of Cancer. Journal International Du Cancer·Thomas G BergerJürgen C Becker

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Citations

Apr 6, 2007·Cancer Immunology, Immunotherapy : CII·Andrew D SimmonsKarin Jooss
Dec 21, 2007·Cancer Immunology, Immunotherapy : CII·Claudia GravekampRobert Reddick
Dec 16, 2006·International Reviews of Immunology·Frédéric Lévy, Sara Colombetti
Apr 2, 2009·European Journal of Immunology·Uta B HofmannAndreas O Eggert
Oct 24, 2009·Experimental Dermatology·Jürgen C BeckerRalph A Reisfeld
Jan 1, 2014·Journal of Immunology Research·Gabriela Piñón-ZárateAndrés Eliú Castell-Rodríguez
Mar 26, 2021·Cancer Immunology, Immunotherapy : CII·Kyp L OxleyGail A Bishop

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