Characterization of mouse serum exosomal small RNA content: The origins and their roles in modulating inflammatory response

Oncotarget
Xin ZhouYuhua Ji

Abstract

In the last decade, although studies on exosomal microRNAs (miRNAs) derived from serum and other body fluids have increased dramatically; the contents and biological significance of serum exosomes under normal conditions remain unclear. In the present study, we profiled the small RNA content of mouse serum exosomes (mSEs) using small RNAseq and found that fragments of transfer RNAs (tRNAs) and miRNAs were the two predominant exosomal RNA species, accounting for approximately 60% and 10% of mapped reads, respectively. Moreover, 466 known and 5 novel miRNAs were identified from two independent experiments, among which the five most abundant miRNAs (miR-486a-5p, miR-22-3p, miR-16-5p, miR-10b-5p and miR-27b-3p) accounted for approximately 60% of all the aligned miRNA sequences. As inferred from the identities of the well known cell- or tissue-specific miRNAs, mSEs were primarily released by RBCs, liver and intestinal cells. Bioinformatics analysis revealed over half of the top 20 miRNAs by abundance were involved in inflammatory responses and further in vitro experiments demonstrated that mSEs potently primed macrophages towards the M2 phenotype. To the best of our knowledge, this is the first study to profile small RNAs from mSEs....Continue Reading

References

Nov 1, 1984·Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire·R M JohnstoneB T Pan
May 15, 2002·Current Biology : CB·Mariana Lagos-QuintanaThomas Tuschl
Aug 3, 2002·Nature Reviews. Immunology·Clotilde ThérySebastian Amigorena
Jan 30, 2008·Current Protocols in Cell Biology·Clotilde ThéryAled Clayton
Feb 23, 2008·Seminars in Cancer Biology·Enikö SonkolyAndor Pivarcsi
Jul 1, 2008·Gynecologic Oncology·Douglas D Taylor, Cicek Gercel-Taylor
Aug 13, 2008·Developmental Cell·Shusheng WangEric N Olson
Nov 13, 2008·PloS One·Melissa Piper HunterClay B Marsh
Nov 26, 2008·Nature Reviews. Immunology·David M Mosser, Justin P Edwards
Dec 26, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Michael W GranerDarell D Bigner
Sep 12, 2009·Clinical Chemistry·Omar F LaterzaWarren E Glaab
Jul 2, 2010·BMC Genomics·Ning-Yi ShaoPhilipp Khaitovich
Sep 17, 2010·Nucleic Acids Research·Yan GaoLinda E Greenbaum
May 28, 2011·The Journal of Allergy and Clinical Immunology·Manish KumarBalaram Ghosh
Aug 9, 2011·Cellular & Molecular Immunology·Md A NahidEdward Kl Chan
Dec 14, 2011·Cancer Prevention Research·Colin C PritchardMuneesh Tewari
Jun 12, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shashi BalaGyongyi Szabo
Jan 18, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Gang Liu, Edward Abraham
May 15, 2013·BMC Genomics·Xiaoyi HuangLiang Wang
Jun 8, 2013·Frontiers in Genetics·Michaela B KirschnerGlen Reid
May 31, 2014·Nucleic Acids Research·Oliver A KentMarc K Halushka
Jun 15, 2014·BMC Genomics·Petra LeidingerAndreas Keller
Jul 16, 2014·Nature Communications·Honghong YaoShilpa Buch
Aug 20, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Mu LiAlexander V Vlassov
Oct 1, 2014·Proceedings of the National Academy of Sciences of the United States of America·John R ChevilletMuneesh Tewari
May 23, 2015·Frontiers in Immunology·Charles Dudley Mills
Jun 19, 2015·Nature Communications·Margaret AlexanderRyan M O'Connell
Aug 25, 2015·Biochimica Et Biophysica Acta·Kobina EssandohGuo-Chang Fan
Nov 11, 2015·Nucleic Acids Research·Yuk Yee LeungLi-San Wang
Dec 10, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Xiaomei TengZhenya Shen
Dec 19, 2015·Annals of Oncology : Official Journal of the European Society for Medical Oncology·F A San LucasH Alvarez

❮ Previous
Next ❯

Citations

Apr 27, 2018·Journal of Extracellular Vesicles·Li ChenDavid R Brigstock
Jun 23, 2020·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Kacoli SenCarlos Rinaldi
Sep 29, 2017·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Mandana PahlavaniNaima Moustaid-Moussa
Oct 18, 2020·Journal of Personalized Medicine·Monika GjorgjievaMichelangelo Foti
May 16, 2019·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mrigya BabutaGyongyi Szabo
Mar 7, 2021·International Journal of Molecular Sciences·Natalia Diaz-GarridoLaura Baldomà

❮ Previous
Next ❯

Methods Mentioned

BETA
RNAseq
chip
PCR
transfection
flow cytometry
FACS
Fluorescence microscopy
proteomic profiling
electron microscopy
electrophoresis

Software Mentioned

Fastx
Bowtie
cutadapt
MiRDeep
Blastn
miRDeep2
IPA
ExoQuick
Randfold
SPSS10

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.