Characterization of mRNA polyadenylation in the apicomplexa

PloS One
Ashley T StevensArthur G Hunt

Abstract

Messenger RNA polyadenylation is a universal aspect of gene expression in eukaryotes. In well-established model organisms, this process is mediated by a conserved complex of 15-20 subunits. To better understand this process in apicomplexans, a group of unicellular parasites that causes serious disease in humans and livestock, a computational and high throughput sequencing study of the polyadenylation complex and poly(A) sites in several species was conducted. BLAST-based searches for orthologs of the human polyadenylation complex yielded clear matches to only two-poly(A) polymerase and CPSF73-of the 19 proteins used as queries in this analysis. As the human subunits that recognize the AAUAAA polyadenylation signal (PAS) were not immediately obvious, a computational analysis of sequences adjacent to experimentally-determined apicomplexan poly(A) sites was conducted. The results of this study showed that there exists in apicomplexans an A-rich region that corresponds in position to the AAUAAA PAS. The set of experimentally-determined sites in one species, Sarcocystis neurona, was further analyzed to evaluate the extent and significance of alternative poly(A) site choice in this organism. The results showed that almost 80% of S. n...Continue Reading

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Citations

Jan 4, 2020·The Journal of Eukaryotic Microbiology·Juan David Ospina-VillaLaurence A Marchat
Aug 22, 2021·Cellular Microbiology·Che Julius NgwaGabriele Pradel
Dec 3, 2021·PLoS Pathogens·Krista FleckVictoria Jeffers

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Datasets Mentioned

BETA
SRR1784218
PRJNA436572

Methods Mentioned

BETA
RNASeq
PAT-Seq
RNA-Seq

Software Mentioned

CLC Genomics Workbench package
TBLASTN
CLC Genomics Workbench
BLAST
BLASTP
Bedtools

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