Characterization of MSCs from human placental decidua basalis in hypoxia and serum deprivation

Cell Biology International
Yong-Can HuangLi Deng

Abstract

Recently, we reported that human PDB (placental decidua basalis) is an excellent source of MSCs (mesenchymal stem cells), meanwhile, PDB-MSCs could survive under hypoxia and serum deprivation. Herein, we investigated the proliferation, clonogentic efficiency, phenotypes, metabolic activity and cytokines secretion of PDB-MSCs in hypoxia and serum deprivation. PDB-MSCs were cultured in four groups: normoxia (20% O2) and complete medium [10% FBS (foetal bovine serum)+DMEM-HG (Dulbecco's modified Eagle's medium-high glucose)], hypoxia and complete medium, normoxia and serum deprivation (0% FBS), and hypoxia and serum deprivation. After 96 h of culture in the above groups, PDB-MSCs maintain the phenotypes stably. Interestingly, hypoxia notably enhanced the proliferation, colony-forming potential and lactate/glucose ratio in complete medium, but suppressed the secretion of BMP-2 (bone morphogenetic protein-2) and bFGF (basic fibroblast growth factor), while it did not change the quantity of VEGF (vascular endothelial growth factor) and bFGF in serum deprivation. Although PDB-MSCs grew slowly and seldom formed a colony unit in hypoxia and serum deprivation, they possessed a moderate metabolism. In conclusion, our results indicate that...Continue Reading

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Citations

Jan 24, 2013·The Spine Journal : Official Journal of the North American Spine Society·Yong-Can HuangKeith D K Luk
Apr 4, 2015·Regenerative Medicine·Alasdair Gawain KayNicholas Robert Forsyth
Jul 16, 2013·Cell Biology International·Suja Ann MathewRamesh Bhonde
Aug 2, 2016·The Tohoku Journal of Experimental Medicine·Qiaoli GuHuilin Yang
Jan 5, 2021·Frontiers in Bioengineering and Biotechnology·Antonietta Rosa SiliniOrnella Parolini

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