Characterization of mucosa-associated bacterial communities in abomasal ulcers by pyrosequencing

Veterinary Microbiology
Alexandra HundThomas Wittek

Abstract

Abomasal ulcers are important pathological alterations of the gastrointestinal tract in cattle and are exceptionally hard to diagnose in vivo. The microbiome of the abomasum in cattle with or without ulcers has hardly been studied to date, and if so, the studies used culture-dependent methods. In the present study, the bacterial communities associated with abomasal ulcers of slaughter cows, bulls, and calves in Austria were described using 16S rRNA gene pyrosequencing. Sequences were clustered into 10,459 operational taxonomic units (OTUs), affiliating to 28 phyla with Proteobacteria, Firmicutes, Bacteroidetes and Tenericutes dominating (96.4% of all reads). The most abundant genera belonged to Helicobacter, Acetobacter, Lactobacillus, and novel Mycoplasma-like phylotypes. Significant differences between the microbial communities of healthy and ulcerated calves compared to cows and bulls could be observed. However, only few statistically significant differences in the abundances of certain OTUs between healthy and ulcerated abomasal mucosa were found. Additionally, near full-length 16S rRNA gene sequences of the most abundant phylotypes were obtained by cloning and Sanger sequencing (n=88). In conclusion, our results allow the ...Continue Reading

References

Jun 1, 1991·Zentralblatt für Veterinärmedizin. Reihe A·U BraunF Ehrensperger
Jul 1, 1990·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·K W MillsA R Doster
Oct 1, 1996·International Journal of Systematic Bacteriology·B PetterssonK E Johansson
Nov 11, 1999·International Journal of Systematic Bacteriology·D De GrooteP Vandamme
Dec 9, 2003·Applied and Environmental Microbiology·Jonas WaldenströmBjörn Olsen
Feb 27, 2004·Nucleic Acids Research·Wolfgang LudwigKarl-Heinz Schleifer
Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Jo Handelsman
Jun 6, 2006·Applied and Environmental Microbiology·G Douglas InglisAnno de Jong
Sep 30, 2006·Folia Microbiologica·E VlkováV Rada
Jun 26, 2007·Applied and Environmental Microbiology·Qiong WangJames R Cole
Oct 13, 2007·Schweizer Archiv für Tierheilkunde·P NebbiaP Robino
Mar 24, 2009·Applied and Environmental Microbiology·Ilias KounatidisKostas Bourtzis
Apr 11, 2009·PLoS Computational Biology·James Robert WhiteMihai Pop
Apr 16, 2009·Clinical Microbiology Reviews·Freddy HaesebrouckRichard Ducatelle
Sep 21, 2010·Applied and Environmental Microbiology·Elena CrottiDaniele Daffonchio
Nov 30, 2012·Nucleic Acids Research·Christian QuastFrank Oliver Glöckner
Jan 26, 2013·The Veterinary Record·B R ValgaerenP R Deprez
May 31, 2013·The ISME Journal·Adam C-N WongAngela E Douglas
Aug 15, 2014·Nature Reviews. Microbiology·Pablo YarzaRamon Rosselló-Móra

❮ Previous
Next ❯

Citations

Feb 18, 2016·International Journal of Food Microbiology·Monika DzieciolStephan Schmitz-Esser
Apr 6, 2016·Veterinary World·A K SharmaC S Randhawa
Mar 30, 2017·Tierärztliche Praxis. Ausgabe G, Grosstiere/Nutztiere·Alexandra Hund, Thomas Wittek
Dec 29, 2018·Journal of Dairy Science·J D BusL E Webb

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.