Characterization of mucosal Candida albicans biofilms

PloS One
Anna Dongari-BagtzoglouJohn Vasilakos

Abstract

C. albicans triggers recurrent infections of the alimentary tract mucosa that result from biofilm growth. Although the ability of C. albicans to form a biofilm on abiotic surfaces has been well documented in recent years, no information exists on biofilms that form directly on mucosal surfaces. The objectives of this study were to characterize the structure and composition of Candida biofilms forming on the oral mucosa. We found that oral Candida biofilms consist of yeast, hyphae, and commensal bacteria, with keratin dispersed in the intercellular spaces. Neutrophils migrate through the oral mucosa and form nests within the biofilm mass. The cell wall polysaccharide beta-glucan is exposed during mucosal biofilm growth and is more uniformly present on the surface of biofilm organisms invading the oral mucosa. We conclude that C. albicans forms complex mucosal biofilms consisting of both commensal bacterial flora and host components. These discoveries are important since they can prompt a shift of focus for current research in investigating the role of Candida-bacterial interactions in the pathogenesis of mucosal infections as well as the role of beta-glucan mediated signaling in the host response.

References

Feb 1, 1979·Journal of Oral Pathology·W R TyldesleyR A Sells
Feb 1, 1990·Acta Odontologica Scandinavica·E Oksala
Dec 1, 1988·The Journal of Antimicrobial Chemotherapy·Frank C Odds
Aug 1, 1986·Canadian Journal of Microbiology·L TrudelM C Lavoie
May 21, 1999·Science·J W CostertonE P Greenberg
Apr 18, 2000·Infection and Immunity·D A ScaramuzzinoD E Bessen
Apr 3, 2002·JAMA : the Journal of the American Medical Association·Garth D EhrlichJ Christopher Post
Jul 27, 2002·Annual Review of Microbiology·Paul StoodleyJ W Costerton
Jan 9, 2003·Journal of Clinical Microbiology·D M KuhnM A Ghannoum
Jun 21, 2003·Journal of Oral Science·Anne M GrimoudGérard Chabanon
Nov 24, 2004·Molecular Microbiology·Deborah A HoganRoberto Kolter
May 24, 2005·Infection and Immunity·Travis S WalkerJerry A Nick
Jul 26, 2005·Infection and Immunity·C C VillarAnna Dongari-Bagtzoglou
Jul 29, 2005·Antimicrobial Agents and Chemotherapy·Elisabeth KugelbergDiarmaid Hughes
Nov 8, 2005·Journal of Clinical Microbiology·Jørn A AasFloyd E Dewhirst
May 3, 2006·PLoS Pathogens·Robert T Wheeler, Gerald R Fink
Jul 4, 2006·Current Opinion in Microbiology·Jeniel E Nett, David R Andes
Jul 13, 2006·JAMA : the Journal of the American Medical Association·Luanne Hall-StoodleyJoseph E Kerschner
Jul 20, 2006·Journal of Medical Microbiology·Mohammed A Al-Fattani, L Julia Douglas
Jan 19, 2007·European Journal of Immunology·Adam D KennedyFrank R DeLeo
May 10, 2007·Nature Protocols·Anna Dongari-Bagtzoglou, Helena Kashleva
Sep 8, 2007·Current Opinion in Otolaryngology & Head and Neck Surgery·J Christopher PostGarth D Ehrlich
Sep 15, 2007·FEMS Immunology and Medical Microbiology·Caroline WestwaterEdward Balish
Nov 17, 2007·The Journal of Infectious Diseases·Neil A R GowBart Jan Kullberg
May 1, 2008·PloS One·Peter SouthernDana A Davis
Sep 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Anton Y PelegEleftherios Mylonakis
Dec 17, 2008·Oral Diseases·C J SeneviratneL P Samaranayake
Mar 31, 2009·Eukaryotic Cell·Emmanouil TampakakisEleftherios Mylonakis

Citations

Dec 30, 2010·Nature Reviews. Microbiology·Jonathan S Finkel, Aaron P Mitchell
May 10, 2011·BMC Microbiology·Zhihong XieAnna Dongari-Bagtzoglou
Mar 10, 2012·Journal of Natural Products·Xiaoru WangRobert H Cichewicz
Oct 4, 2012·The Journal of Infectious Diseases·Zhihong XieAnna Dongari-Bagtzoglou
Nov 22, 2013·PloS One·Lan-Xue ZhaoYuan-Ying Jiang
Nov 29, 2011·International Journal of Microbiology·Alexandra Brand
Dec 14, 2011·International Journal of Microbiology·Hélène Tournu, Patrick Van Dijck
Oct 4, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Jurjen ter MaatHan Zuilhof
Aug 27, 2013·Current Genetics·Lotte Mathé, Patrick Van Dijck
Dec 19, 2015·FEMS Yeast Research·Christophe d'Enfert, Guilhem Janbon
Jan 26, 2016·Microbes and Infection·Megha Gulati, Clarissa J Nobile
Jan 23, 2016·Biofouling·Yuri Wanderley CavalcantiDavid W Williams
Aug 23, 2011·Trends in Microbiology·M M Harriott, Mairi C Noverr
Sep 8, 2010·Journal of Microbiological Methods·Tom Coenye, Hans J Nelis
Mar 6, 2016·Archives of Oral Biology·Yuri Wanderley CavalcantiWander José da Silva
Mar 15, 2016·Journal of Basic Microbiology·Thanwa WongsukNatthanej Luplertlop
Mar 22, 2016·Pathogens·Jeniel E Nett
Nov 15, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Aspasia KatragkouThomas J Walsh
Aug 14, 2010·Microbiology·M M HarriottMairi C Noverr
Aug 25, 2015·FEMS Yeast Research·Lindsay E O'DonnellGordon Ramage
Jul 12, 2011·Current Opinion in Microbiology·Shantanu Ganguly, Aaron P Mitchell
May 2, 2015·Plastic and Reconstructive Surgery·Zoe Dorothea PanaEmmanuel Roilides
May 12, 2015·International Journal of Medical Microbiology : IJMM·Josefine HirschfeldIsabelle Bekeredjian-Ding
Feb 24, 2015·Cellular and Molecular Life Sciences : CMLS·Elizabeth J PolviLeah E Cowen
Feb 14, 2012·Journal of Ethnopharmacology·Mohd Sajjad Ahmad Khan, Iqbal Ahmad
May 30, 2013·Virulence·Anna C B P CostaAntonio Olavo Cardoso Jorge
Aug 15, 2014·Frontiers in Cellular and Infection Microbiology·Patricia I DiazAnna Dongari-Bagtzoglou
Apr 26, 2016·Oral Diseases·W Nittayananta
Jun 1, 2016·Frontiers in Microbiology·Daniel Montelongo-JaureguiJose L Lopez-Ribot
Apr 13, 2012·PLoS Pathogens·Saranna Fanning, Aaron P Mitchell
Oct 22, 2015·Annual Review of Microbiology·Clarissa J Nobile, Alexander D Johnson
Jun 6, 2013·PloS One·Hongbin XuAnna Dongari-Bagtzoglou
Jan 5, 2017·Journal of Medicinal Chemistry·Shanchao WuChunquan Sheng
Jul 14, 2017·PloS One·Simona PauloneSamuele Peppoloni
Mar 1, 2018·Frontiers in Medicine·Mafalda Cavalheiro, Miguel Cacho Teixeira
Oct 25, 2017·Nature Reviews. Microbiology·Matthew B LohseClarissa J Nobile
Jun 13, 2017·Journal of Dental Research·K EllepolaC J Seneviratne
Jan 27, 2018·Clinical Oral Investigations·Mariella Vieira Pereira LeãoAntonio Olavo Cardoso Jorge
Jun 3, 2016·Microbiology and Molecular Biology Reviews : MMBR·David R Soll, Karla J Daniels
May 1, 2012·Eukaryotic Cell·Saranna FanningAaron P Mitchell
Sep 16, 2015·Infection and Immunity·Jeniel E NettDavid R Andes
Nov 23, 2011·Infection and Immunity·Patricia I DiazAnna Dongari-Bagtzoglou
Nov 26, 2014·Infection and Immunity·Eric F KongMary Ann Jabra-Rizk
Sep 4, 2014·Infection and Immunity·Jeniel E NettDavid R Andes
Apr 23, 2019·PLoS Pathogens·Martinna BertoliniAnna Dongari-Bagtzoglou
Oct 25, 2013·Journal of Oral Microbiology·David W WilliamsMichael A O Lewis
Feb 4, 2014·Journal of Oral Microbiology·Austin RickerAnna Dongari-Bagtzoglou
Dec 20, 2014·Journal of Oral Microbiology·Josefine Hirschfeld
Apr 11, 2016·Cellular Microbiology·Pedro Miramón, Michael C Lorenz
Mar 1, 2020·Applied and Environmental Microbiology·João Gabriel Silva SouzaAnna Dongari-Bagtzoglou
Feb 3, 2017·Frontiers in Cellular and Infection Microbiology·Shankar ThangamaniMohamed N Seleem
Apr 30, 2016·The Journal of Biological Chemistry·Donald C Sheppard, P Lynne Howell
Jan 27, 2018·Journal of Fungi·Caroline B Costa-OrlandiMaria José S Mendes-Giannini
Jan 27, 2018·Journal of Fungi·Andrew McCall, Mira Edgerton
Jun 6, 2018·Frontiers in Microbiology·Shankargouda PatilKamran H Awan
Sep 25, 2019·Journal of Fungi·Martinna Bertolini, Anna Dongari-Bagtzoglou
Jan 30, 2018·Frontiers in Immunology·John F KernienJeniel E Nett
Feb 12, 2020·The ISME Journal·João Gabriel Silva SouzaAnna Dongari-Bagtzoglou
Jan 27, 2018·Journal of Fungi·Sónia SilvaMariana Henriques
Jul 18, 2017·BioMed Research International·Leila Maria Marchi-AlvesIsabel Amélia Costa Mendes
Oct 13, 2018·Biomicrofluidics·Christopher RahimiChristopher B Raub
Nov 5, 2019·Frontiers in Microbiology·Karen G N OshiroOctávio Luiz Franco
Dec 1, 2020·Future Microbiology·Lucia Černáková, Célia F Rodrigues
Jan 28, 2021·Journal of Fungi·Eunae ChoHyung-Joon Ahn
Jan 23, 2021·Critical Reviews in Microbiology·Nicole O PondeJonathan P Richardson
Feb 5, 2021·Expert Opinion on Pharmacotherapy·Liliana ScorzoniEleftherios Mylonakis
Nov 13, 2020·Frontiers in Microbiology·Lariane Teodoro OliveiraMaria José Soares Mendes Giannini
Mar 20, 2021·Archives of Oral Biology·Claudia Viviane Guimarães PellissariWalter Luiz Siqueira
Jul 16, 2013·Archives of Oral Biology·Luis Octavio Sánchez-VargasRaimundo Rivas-Caceres
Nov 30, 2020·Experimental Eye Research·Bethany MillsN Venkatesh Prajna

Methods Mentioned

BETA
confocal microscopy

Related Concepts

Candida albicans
Candidiasis
Cell Motility
Cell Wall
Keratin
Buccal Mucosa
Muscularis Mucosae
Neutrophil Band Cells
Polysaccharides
Signal Transduction

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