Characterization of Müller glia and neuronal progenitors during adult zebrafish retinal regeneration.

Experimental Eye Research
Ryan ThummelD R Hyde

Abstract

The adult zebrafish retina exhibits a robust regenerative response following light-induced photoreceptor cell death. This response is initiated by the Müller glia proliferating in the inner nuclear layer (INL), which gives rise to neuronal progenitor cells that continue to divide and migrate to the outer nuclear layer (ONL), where they differentiate into rod and cone photoreceptors. We previously conducted a microarray analysis of retinal gene expression at 16, 31, 51, 68, and 96 h of constant intense-light treatment to identify genes and their corresponding proteins that may be involved in the generation and proliferation of the neuronal progenitor cells. We examined the expression of two candidate transcription factors, Pax6 and Ngn1, and one candidate transgene, olig2:EGFP, in the regenerating light-damaged retina. We compared the temporal and spatial expression patterns of these markers relative to PCNA (proliferating cell nuclear antigen), an established marker for proliferating cells in the zebrafish retina, and the Tg(gfap:EGFP) nt11 transgenic line that specifically labels Müller glial cells. We found that Müller glial cells dedifferentiate during regeneration, based on the loss of cell-specific markers such as GFAP (gl...Continue Reading

References

Aug 1, 1965·Developmental Biology·J L Coulombre, A J Coulombre
Jan 1, 1995·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·W C Michel, L M Lubomudrov
Jan 19, 1999·Methods in Cell Biology·H W DetrichL I Zon
Jan 26, 2000·Development, Growth & Differentiation·Y KanekoY Hanyu
Aug 9, 2002·Developmental Biology·Hae-Chul ParkBruce Appel
Sep 19, 2003·Acta histochemica·Dong Hoon ShinJong Wan Kim
Jan 24, 2004·Methods in Cell Science : an Official Journal of the Society for in Vitro Biology·Jimann ShinBruce Appel
Apr 15, 2004·Molecular and Cellular Neurosciences·M A HackM Götz
Apr 20, 2004·Progress in Retinal and Eye Research·Peter HitchcockDeborah Otteson
Jul 29, 2004·The Journal of Comparative Neurology·Peter Hitchcock, Laura Kakuk-Atkins
Oct 22, 2004·Development·Patrick BladerUwe Strähle
Dec 22, 2004·Progress in Retinal and Eye Research·Andy J Fischer
Dec 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·Annalisa BuffoMagdalena Götz
May 3, 2006·Glia·Keith L LigonDavid H Rowitch
Jun 10, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Blake V Fausett, Daniel Goldman
Jul 29, 2006·BMC Developmental Biology·Pamela A RaymondJohn J Perkowski
Feb 16, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shane M FimbelDavid R Hyde
Jun 29, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Rebecca L BernardosPamela A Raymond
Nov 15, 2007·Developmental Neurobiology·Tshering SherpaDeborah L Stenkamp
Dec 29, 2007·Developmental Neurobiology·Ryan ThummelDavid R Hyde

❮ Previous
Next ❯

Citations

Oct 26, 2010·Cellular and Molecular Life Sciences : CMLS·Susan E Brockerhoff, James M Fadool
Apr 12, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Craig M NelsonDavid R Hyde
Jan 11, 2012·Journal of Visualized Experiments : JoVE·Ryan ThummelDavid R Hyde
Apr 11, 2014·Development·Alexandra D AlmeidaWilliam A Harris
Dec 10, 2013·Journal of Neuroscience Methods·Alexis Tapanes-CastilloJeffery A Plunkett
Jan 15, 2014·Progress in Retinal and Eye Research·Jenny R Lenkowski, Pamela A Raymond
Mar 1, 2016·Frontiers in Cellular Neuroscience·Vidhyasankar KrishnamoorthyNarender K Dhingra
Dec 1, 2015·Experimental Eye Research·Stephen G WilsonAnn C Morris
Oct 21, 2015·Proceedings of the National Academy of Sciences of the United States of America·Yumi UekiThomas A Reh
Feb 10, 2016·Frontiers in Neuroanatomy·Blanca Fernández-LópezMaría Celina Rodicio
Dec 18, 2013·Translational Research : the Journal of Laboratory and Clinical Medicine·Honghua YuDong Feng Chen
Apr 12, 2015·Science China. Life Sciences·WenChao ShiLingFei Luo
Sep 29, 2011·Experimental Eye Research·Zhao QinRyan Thummel
Jul 22, 2010·Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie·Brian G Ballios, Derek van der Kooy
Nov 10, 2009·Experimental Eye Research·Elena CidAlmudena Velasco
Apr 22, 2009·Experimental Eye Research·Ruth Bejarano-EscobarJavier Francisco-Morcillo
Oct 24, 2015·Experimental Eye Research·Jennifer L ThomasRyan Thummel
Jan 17, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Sherry G ClendenonJames A Marrs
Sep 30, 2010·The Journal of Comparative Neurology·Rajesh RamachandranDaniel Goldman
Jun 16, 2010·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Ruth Bejarano-EscobarJavier Francisco-Morcillo
Jan 28, 2014·British Journal of Pharmacology·E GramageP Hitchcock
Jul 25, 2013·Experimental Eye Research·Ryne A Gorsuch, David R Hyde
Oct 6, 2012·The Biochemical Journal·Karla Barbosa-SabaneroKatia Del Rio-Tsonis
Jul 12, 2011·Progress in Retinal and Eye Research·Deborah L Stenkamp
Mar 20, 2012·Experimental Eye Research·Jennifer L ThomasRyan Thummel
May 27, 2014·Developmental Biology·Kamya RajaramJames G Patton
Dec 22, 2014·Comptes rendus biologies·Bice AvalloneChiara Maria Motta
Jul 6, 2016·Frontiers in Neuroanatomy·Nuria Sánchez-Farías, Eva Candal
Apr 14, 2016·Development·Katharina LustJoachim Wittbrodt
Oct 12, 2018·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Alaa El-Din H SayedHiroshi Mitani
Sep 21, 2011·The European Journal of Neuroscience·Günther K H Zupanc, Ruxandra F Sîrbulescu

❮ Previous
Next ❯

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