PMID: 8466647Apr 1, 1993Paper

Characterization of multiple transcription initiation sites of the ovine insulin-like growth factor-I gene and expression profiles of three alternatively spliced transcripts

DNA and Cell Biology
S M OhlsenE A Wong

Abstract

Alternative splicing of ovine insulin-like growth factor-I (IGF-I) transcripts generates three different mRNAs. Class 1 and class 2 transcripts contain exons 1 and 2 spliced to exon 3, respectively. A novel IGF-I mRNA containing exon W is spliced to exon 3 and has been located upstream of exon 1. No in-frame methionine codon was present in exon W and therefore translation is proposed to initiate at the methionine codon present in exon 3. Using primer extension, transcription initiation sites were found 179, 336, and 368 nucleotides upstream of exon 1 and 86, 96, 131, and approximately 850 nucleotides upstream of exon 2. The locations of these transcription initiation sites are well conserved among mammalian and avian IGF-I genes. Expression of exon 1-, 2-, and W-specific transcripts was examined in brain, heart, kidney, liver, lung, skeletal muscle, and spleen from adult ewes or 75-day fetal lambs using a reverse transcriptase-polymerase chain reaction assay. Exon 1 transcripts were the most abundant and found in all fetal and adult tissues. Exon 2 transcripts were found in all tissues and were generally expressed the highest in adult liver. Exon W transcripts were also found to be expressed in all tissues examined. Thus, the t...Continue Reading

References

Dec 1, 1991·Journal of Molecular Endocrinology·J C SaundersR S Gilmour
Dec 1, 1991·Molecular Endocrinology·S W KimP Rotwein
Jun 11, 1991·Molecular and Cellular Endocrinology·E JansenJ S Sussenbach
Mar 15, 1991·Biochemical and Biophysical Research Communications·P H SteenberghJ S Sussenbach
Apr 30, 1991·Biochemical and Biophysical Research Communications·M L AdamoC T Roberts
Apr 7, 1989·Cell·S T Smale, D Baltimore
Jan 20, 1986·FEBS Letters·P de Pagter-HolthuizenJ S Sussenbach
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·W L LoweD LeRoith

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Citations

Jun 1, 1994·Molecular and Cellular Endocrinology·D RamagnoloJ D Turner
May 1, 1994·Molecular and Cellular Endocrinology·R ZarrilliA Riccio
Sep 4, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Darran N ToshMina Desai
May 15, 2013·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Jinyu ZhangZhihui Zhao
Aug 28, 2015·Journal of Developmental Origins of Health and Disease·D J CarrJ M Wallace
Aug 7, 1998·Journal of Pediatric Surgery·K K NobuharaJ M Wilson

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