PMID: 16538027Mar 16, 2006Paper

Characterization of muscarinic receptor-mediated cationic currents in longitudinal smooth muscle cells of mouse small intestine

Journal of Pharmacological Sciences
Takashi SakamotoSeiichi Komori

Abstract

In mouse intestinal smooth muscle cells held at -50 mV, carbachol evoked an atropine-sensitive inward current in the intracellular presence of Cs(+). The current response consisted of an initial peak followed by a smaller plateau component on which oscillatory currents frequently arose. Results from various experimental procedures indicated that the inward current is a muscarinic receptor-operated cationic current (mI(cat)) sensitive to cytosolic Ca(2+) concentration ([Ca(2+)](i)) and that the initial peak and oscillatory components are contaminated by Ca(2+)-activated Cl(-) currents. Under conditions of [Ca(2+)](i) buffered to 100 nM, the mI(cat) response to cumulative carbachol applications was inhibited competitively by an M(2)-selective antagonist but non-competitively by an M(3)-selective one. Also it was severely reduced by pertussis toxin (PTX) treatment or a phospholipase C (PLC) inhibitor. Comparative analysis of mI(cat) in mouse and guinea-pig intestinal myocytes indicated that the underlying channels resemble between those myocytes in agonist sensitivity, current-voltage relationship, and unitary conductance. The results suggest that in mouse intestinal myocytes, mI(cat) arises mainly via an M(2)/M(3) synergistic mec...Continue Reading

References

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Citations

Jun 25, 2009·Gastroenterology·Volodymyr V TsvilovskyyVeit Flockerzi
Oct 11, 2008·European Journal of Pharmacology·Takashi SakamotoToshihiro Unno
Mar 13, 2016·Journal of Ethnopharmacology·Hyun Jung KimByung Joo Kim
Nov 7, 2008·Journal of Pharmacological Sciences·Masayuki YamamotoSeiichi Komori
Oct 27, 2015·The Journal of Veterinary Medical Science·Yasuyuki TanahashiSeiichi Komori
Dec 1, 2013·Journal of Pharmacopuncture·Byung Joo Kim
Jan 23, 2021·International Journal of Molecular Sciences·Yasuyuki TanahashiToshihiro Unno

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