Characterization of Mutations That Affect the Nonoxidative Pentose Phosphate Pathway in Sinorhizobium meliloti

Journal of Bacteriology
Justin P HawkinsIvan J Oresnik

Abstract

Sinorhizobium meliloti is a Gram-negative alphaproteobacterium that can enter into a symbiotic relationship withMedicago sativaandMedicago truncatulaPrevious work determined that a mutation in thetkt2gene, which encodes a putative transketolase, could prevent medium acidification associated with a mutant strain unable to metabolize galactose. Since the pentose phosphate pathway inS. melilotiis not well studied, strains carrying mutations in eithertkt2andtal, which encodes a putative transaldolase, were characterized. Carbon metabolism phenotypes revealed that both mutants were impaired in growth on erythritol and ribose. This phenotype was more pronounced for thetkt2mutant strain, which also displayed auxotrophy for aromatic amino acids. Changes in pentose phosphate pathway metabolite concentrations were also consistent with a mutation in eithertkt2ortalThe concentrations of metabolites in central carbon metabolism were also found to shift dramatically in strains carrying atkt2mutation. While the concentrations of proteins involved in central carbon metabolism did not change significantly under any conditions, the levels of those associated with iron acquisition increased in the wild-type strain with erythritol induction. These...Continue Reading

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Citations

Sep 12, 2018·Canadian Journal of Microbiology·George C diCenzoAlessio Mengoni
Jan 17, 2019·Preparative Biochemistry & Biotechnology·Karuna L Sorde, Laxmi Ananthanarayan

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