Characterization of Mycoplasmatales virus-laidlawii 3.

The Journal of General Virology
D J GarwesR N Gourlay

Abstract

Purified preparations of Mycoplasmatales virus-laidlawii 3 were negatively stained and studied by electron microscopy. They were seen to consist of uniform sized particles having a polyhedral head, 57 nm by 61 nm, and a short tail, 25 nm long, joined to the head at one vertex by a collar. The particles were shown to have buoyant densities of 1-477 g/ml in CsC1, 1-32 g/ml and 1-26 g/ml in potassium tartrate and a sedimentation coefficient, S20,W, OF 290 +/- 13. They are composed of 35-2% double-stranded DNA and five structural polypeptides with approximate mol. wt. of 172000, 81000 73000, 68000 and 43000. The classification of the virus from its morphology and chemical properties is discussed.

Citations

Jan 1, 1988·Critical Reviews in Microbiology·J Maniloff
Apr 1, 1978·Infection and Immunity·P S Lombardi, B C Cole
Aug 1, 1976·Journal of Bacteriology·J A NowakJ Maniloff

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