Characterization of NAD uptake in mammalian cells

The Journal of Biological Chemistry
Richard A BillingtonArmando A Genazzani

Abstract

Recent evidence has shown that NAD(P) plays a variety of roles in cell-signaling processes. Surprisingly, the presence of NAD(P) utilizing ectoenzymes suggests that NAD(P) is present extracellularly. Indeed, nanomolar concentrations of NAD have been found in plasma and other body fluids. Although very high concentrations of NAD have been shown to enter cells, it is not known whether lower, more physiological concentrations are able to be taken up. Here we show that two mammalian cell types are able to transport low NAD concentrations effectively. Furthermore, extracellular application of NAD was able to counteract FK866-induced cell death and restore intracellular NAD(P) levels. We propose that NAD uptake may play a role in physiological NAD homeostasis.

References

Aug 15, 1997·The Biochemical Journal·G M Cohen
Aug 26, 1998·Biochimica Et Biophysica Acta·J CaiD P Jones
Nov 7, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L FrancoA De Flora
Feb 11, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E ZocchiA De Flora
Mar 11, 2000·European Journal of Biochemistry·M Ziegler
Sep 28, 2000·Biochemical and Biophysical Research Communications·R A Billington, A A Genazzani
Dec 2, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S Bruzzone De Flora A
Aug 21, 2003·Biochemical and Biophysical Research Communications·Weihai YingRaymond A Swanson
Jan 6, 2004·Cellular and Molecular Life Sciences : CMLS·G MagniS Ruggieri
Mar 9, 2004·Trends in Biochemical Sciences·Felicitas BergerMathias Ziegler
Mar 19, 2004·The Journal of Biological Chemistry·Lucrezia GuidaAntonio De Flora
Sep 15, 2004·The Journal of Biological Chemistry·Lisa M SmythVioleta N Mutafova-Yambolieva
Aug 3, 2005·The Journal of Biological Chemistry·Antje C HeidemannHelmut Kettenmann
Oct 27, 2005·Journal of Molecular Modeling·Neil J Harrison, Sarah C R Lummis
Jan 13, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R A BillingtonA A Genazzani
Jun 20, 2006·Nature Structural & Molecular Biology·Javed A KhanLiang Tong
Aug 24, 2006·The Journal of Biological Chemistry·Iliana MoreschiAntonio De Flora
Dec 13, 2006·Trends in Biochemical Sciences·Peter BelenkyCharles Brenner
Feb 3, 2007·Current Opinion in Gastroenterology·Javier R RevolloShin-ichiro Imai
Mar 24, 2007·Molecular Medicine·Hon Cheung Lee
Mar 24, 2007·Molecular Medicine·Richard A BillingtonElena Zocchi
May 1, 2007·Expert Opinion on Therapeutic Targets·Javed A KhanLiang Tong
Jun 23, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maria Thereza P BarbosaEduardo Nunes Chini

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Citations

May 30, 2009·The Journal of Biological Chemistry·Tong ZhangW Lee Kraus
Feb 26, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Conrad C AlanoRaymond A Swanson
Sep 9, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Olga M Ocón-GroveRamesh Ramachandran
Jan 15, 2014·Rejuvenation Research·Andrew R Mendelsohn, James W Larrick
Feb 25, 2009·The Journal of Cell Biology·Laura ConfortiMichael Coleman
Apr 4, 2015·Critical Reviews in Biochemistry and Molecular Biology·Andrey NikiforovMathias Ziegler
Feb 25, 2015·Pharmacology & Therapeutics·Deepak SampathPeter S Dragovich
May 12, 2009·Biochemical Pharmacology·Laura FormentiniAlberto Chiarugi
Sep 19, 2008·The Plant Journal : for Cell and Molecular Biology·Xudong Zhang, Zhonglin Mou
Jan 27, 2012·The European Journal of Neuroscience·Leonie DurninVioleta N Mutafova-Yambolieva
Sep 12, 2015·Pigment Cell & Melanoma Research·Ambra A GrollaCristina Travelli
Jun 3, 2014·Pharmacology & Therapeutics·Violeta N Mutafova-Yambolieva, Leonie Durnin
Apr 23, 2015·The International Journal of Biochemistry & Cell Biology·Alla B SalminaLyudmila L Petrova
Oct 28, 2008·Current Biology : CB·Lianne C DavisAntony Galione
Oct 18, 2014·Cell Death and Differentiation·M Di StefanoL Conforti
Jan 18, 2018·Journal of Cellular Physiology·Hong-Li XieJing-He Tan
Aug 21, 2018·Rejuvenation Research·James ClementNady Braidy
Feb 21, 2019·Antioxidants & Redox Signaling·Chaitanya A Kulkarni, Paul S Brookes
Oct 18, 2008·Physiology·Wei-Lien Yen, Daniel J Klionsky
Jun 26, 2009·Critical Reviews in Clinical Laboratory Sciences·Alfred J Meijer, Patrice Codogno
Apr 28, 2020·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Giorgia ColomboCristina Travelli
Jan 20, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Daniel S MatasicBarry London
Dec 5, 2019·Metabolites·Veronika KulikovaAndrey Nikiforov
Sep 16, 2011·Molecular Pharmacology·Maria PittelliAlberto Chiarugi
Oct 31, 2014·The Journal of Biological Chemistry·Christopher Del NagroThomas O'Brien
Aug 31, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Iris GehrkeVersha Banerji
Jan 2, 2021·Signal Transduction and Targeted Therapy·Lola E Navas, Amancio Carnero
Dec 8, 2009·Journal of Medicinal Chemistry·Giampiero ColombanoArmando A Genazzani
Jun 8, 2021·The Journal of Biological Chemistry·Daniela BuonvicinoAlberto Chiarugi

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