Characterization of naturally occurring CD4+CD25+ regulatory T cells in rhesus monkeys

Transplantation
K G HaanstraM Jonker

Abstract

Translational research in a relevant preclinical model is recommended before Treg-inducing protocols can be implemented in humans. We have characterized rhesus monkey CD25 cells phenotypically and functionally. The phenotype of CD4(+)CD25(high) cells was determined by FACS, focusing on established markers of mouse and human Treg cells. Percentages of cells positive for CD45RA, CD62L, and intracellular CTLA-4 and FOXP3 were compared between CD4(+)CD25(high) and CD4(+)CD25(-) cells. CD25 cells stimulated with anti-CD3, ConA, and/or allogeneic peripheral blood mononuclear cells were mixed with freshly isolated CD25 cells. The suppressive activity of the CD25 cells in vitro was assessed using several experimental conditions. Rhesus monkey CD4(+)CD25(high) cells expressed high intracellular levels of CTLA-4 and FOXP3, whereas expression was negligible in CD4(+)CD25(-) cells. The CD25(high) population was mostly CD45RA(-), indicative of a memory phenotype. The CD25(+) cells were anergic, because they showed low proliferative responses, no interleukin-2 production, and some interferon-gamma and interleukin-10 production. Proliferation of CD4(+)CD25(-) cells stimulated by anti-CD3 or allogeneic cells was decreased when CD4(+)CD25(-) ce...Continue Reading

References

Apr 12, 2001·European Journal of Immunology·L A StephensF Powrie
Jul 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·C Baecher-AllanD A Hafler
Jan 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cherry I KingsleyKathryn J Wood
Jun 6, 2002·Immunology·Kajsa WingElisabeth Suri-Payer
Aug 7, 2002·The Journal of Experimental Medicine·Francesco AnnunziatoSergio Romagnani
Mar 4, 2003·Nature Immunology·Jason D FontenotAlexander Y Rudensky
Mar 18, 2003·Transplantation·Krista G HaanstraMargreet Jonker
Jun 19, 2003·The Journal of Clinical Investigation·David H Sachs
May 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ismé M de KleerBerent Prakken
Jun 9, 2004·Drug Discovery Today·Bert A 't HartMargreet Jonker
Dec 29, 2004·Human Immunology·Mary E MorganRené E M Toes
Jun 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hans J P M KoenenIrma Joosten
Jul 12, 2005·The Journal of Clinical Investigation·Danila ValmoriMaha Ayyoub
Dec 8, 2005·Blood·Nabila SeddikiBarbara Fazekas de Saint Groth
Dec 13, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Clement K AsieduJudith M Thomas
Apr 14, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·L S KeanC P Larsen
Apr 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Marc A GavinAlexander Y Rudensky
Sep 7, 2006·The Journal of Clinical Investigation·Milica Vukmanovic-StejicArne N Akbar
Oct 20, 2006·European Journal of Immunology·Alison H BanhamElisabeth Suri-Payer
Dec 13, 2006·European Journal of Immunology·Jun WangRené E M Toes
Feb 13, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J H L VelthuisC C Baan
Aug 24, 2007·Transplantation·Elaine Long, Kathryn J Wood
Sep 12, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·K G HaanstraM Jonker

❮ Previous
Next ❯

Citations

Mar 16, 2013·Nature Reviews. Drug Discovery·Guillaume VaquerBruno Sepodes
Feb 4, 2012·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·K SinghL S Kean
Jan 16, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Krista G HaanstraBernard Vanhove
Nov 19, 2017·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Hwajung KimKyung-Suk Suh
Oct 13, 2020·Renal Failure·Balamurugan PackialakshmiXiaoming Zhou
Feb 3, 2021·Proceedings of the National Academy of Sciences of the United States of America·Melba MuñozMax Löhning
Jul 3, 2021·Frontiers in Cell and Developmental Biology·Angus W ThomsonMohamed B Ezzelarab

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Related Papers

American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons
K G HaanstraM Jonker
The New England Journal of Medicine
Ganesh SuntharalingamNicki Panoskaltsis
© 2022 Meta ULC. All rights reserved