Characterization of near full-length genomes of HIV type 1 strains in Denmark: basis for a universal therapeutic vaccine

AIDS Research and Human Retroviruses
Betina S AndresenAnders Fomsgaard

Abstract

We report here the near full-length sequence characterization of 17 Danish clinical HIV-1 strains isolated from HLA-A02 patients not in need of ART, with relatively low viral loads and normal CD4 cell counts. Sequencing was performed directly on DNA extracted from short-term cocultures of PBMCs. The near full-length genomes did not contain any major insertions, deletions, or rearrangements. Sixteen of the isolates were characterized as nonrecombinant subtype B and one isolate as nonrecombinant subtype C. Phylogenetic analysis did not reveal any founder effect among the sequences. Also, we investigated the presence of infrequently targeted subdominant HLA-A02-binding CTL epitopes. The epitopes were conserved in the Danish strains as well as globally in reference sequences of all subtypes. Thus, the selected epitopes were not subtype-specific or region-specific. This lends support for the concept of a universal immunotherapeutic vaccine construct based on these epitopes.

References

Jan 10, 2001·The Journal of Experimental Medicine·P J GoulderC Brander
Jan 16, 2004·Scandinavian Journal of Infectious Diseases·Louise B JørgensenClaus Nielsen
Apr 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·June Kan-MitchellMichal Bereta
Jul 21, 2004·Briefings in Bioinformatics·Sudhir KumarMasatoshi Nei
Mar 23, 2006·Annual Review of Immunology·Andrew J McMichael

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Citations

Jan 15, 2013·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Ingrid KarlssonAnders Fomsgaard
Jul 14, 2011·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Lasse VinnerAnders Fomsgaard
Nov 30, 2016·International Journal of Molecular Sciences·Antonio Victor Campos CoelhoSergio Crovella

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