Characterization of neuromas in peripheral nerves and their effects on heterotopic bone formation

Molecular Pain
Jordan MinarelliAlan R Davis

Abstract

The formation of neuromas involves expansion of the cellular components of peripheral nerves. The onset of these disorganized tumors involves activation of sensory nerves and neuroinflammation. Particularly problematic in neuroma is arborization of axons leading to extreme, neuropathic pain. The most common sites for neuroma are the ends of transected nerves following injury; however, this rodent model does not reliably result in neuroma formation. In this study, we established a rodent model of neuroma in which the sciatic nerve was loosely ligated with two chromic gut sutures. This model formed neuromas reliably (∼95%), presumably through activation of the neural inflammatory cascade. Resulting neuromas had a disorganized structure and a significant number of replicating cells. Quantification of changes in perineurial and Schwann cells showed a significant increase in these populations. Immunohistochemical analysis showed the presence of β-tubulin 3 in the rapidly expanding nerve and a decrease in neurofilament heavy chain compared to the normal nerve, suggesting the axons forming a disorganized structure. Measurement of the permeability of the blood-nerve barrier shows that it opened almost immediately and remained open as l...Continue Reading

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Citations

Oct 6, 2020·Veterinary Pathology·Lisa G LaniganBrad Bolon

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Methods Mentioned

BETA
amputation
flow cytometry
biopsies
confocal microscopy

Software Mentioned

CTAn
CatWalk
BD FACSDiva
XT
CTVox
CatWalk Automated Gait Analysis System

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