Characterization of neutral amino acid transport in a marine pseudomonad.

Journal of Bacteriology
J E Fein, R A MacLeod

Abstract

The transport of neutral amino acids in marine pseudomonad B-16 (ATCC 19855) has been investigated. From patterns of competitive inhibition, mutant analysis, and kinetic data, two active transport systems with overlapping substrate specificities were distinguished and characterized. One system (DAG) served glycine, D-alanine, D-serine, and alpha-aminoisobutyric acid (AIB) and, to a lesser extent, L-alanine and possibly other related neutral D- and L-amino acids. The other system (LIV) showed high stereospecificity for neutral amino acids with the L configuration and served primarily to transport L-leucine, L-isoleucine, L-valine, and L-alanine. This system exhibited low affinity for alpha-aminoisobutyric acid. Neither system was able to recognize structural analogues with modified alpha-amino or alpha-carboxyl groups. The kinetic parameters for L-alanine transport by the DAG and LIV systems were determined with appropriate mutants defective in either system. For L-alanine, Kt values of 4.6 X 10(-5) and 1.9 X 10(-4) M and Vmax values of 6.9 and 20.8 nmol/min per mg of cell dry weight were obtained for transport via the DAG and LIV systems respectively. alpha-Aminoisobutyric acid transport heterogeneity was also resolved with the...Continue Reading

References

Jan 1, 1975·Journal of Bacteriology·H M Hassan, R A MacLeod
May 25, 1973·Biochimica Et Biophysica Acta·J T Holden, J M Bunch
May 1, 1973·Journal of Bacteriology·S D Cosloy
May 1, 1973·Journal of Bacteriology·S D Cosloy, E McFall
Oct 1, 1973·Journal of Bacteriology·J C Robbins, D L Oxender
Dec 1, 1973·Journal of Bacteriology·M RahmanianD L Oxender
Jun 1, 1973·Canadian Journal of Microbiology·J A GowR A MacLeod
Jul 1, 1973·Journal of Bacteriology·S L Tang, D H Howard
May 1, 1974·Journal of Bacteriology·J Reizer, N Grossowicz
Aug 1, 1962·Archives of Biochemistry and Biophysics·T D BROCK, G MOO-PENN
Mar 1, 1965·Biochemistry·D KESSEL, M LUBIN
Mar 1, 1952·Journal of Bacteriology·J LEDERBERG, E M LEDERBERG

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Citations

Dec 1, 1984·Applied and Environmental Microbiology·T T Eighmy, P L Bishop
Mar 1, 1986·Journal of Bacteriology·P R MacLeod, R A MacLeod
Dec 1, 1995·Applied and Environmental Microbiology·C B MiguezR A Macleod
Jul 1, 1982·Journal of Bacteriology·J Lee-Kaden, W Simonis
Apr 1, 1977·Journal of Bacteriology·S M PearceT Lee

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