PMID: 7018580Apr 14, 1981Paper

Characterization of neutral isozymes of human alpha-glucosidase: differences in substrate specificity, molecular weight and electrophoretic mobility

Biochimica Et Biophysica Acta
F Martiniuk, R Hirschhorn

Abstract

We have previously defined two isozymes of neutral alpha-glucosidase (alpha-D-glucoside glucohydrolase, EC 3.2.1.20) on the basis of differences in electrophoretic mobility and designated these neutral alpha-glucosidase AB and alpha-glucosidase C (Swallow, D.M., Corney, G., Harris, H. and Hirschhorn, R. (1975) Ann. Hum. Gen. 38, 391-406). We now describe differences between the two isozymes with respect to molecular weight, solubility in (NH4)2SO4, glycosylation, isoelectric point and substrate specificities. Neutral alpha-glucosidase C is precipitable in 40-60% (NH4)2SO4, has a molecular weight of 92 000, an isoelectric point of 5.5 and releases glucose from glycogen as well as from low molecular weight artificial and natural substrates containing alpha 1-4 glucosidic linkages. Neutral alpha-glucosidase AB precipitates at 0-40% (NH4)2SO4, binds to concanavalin A, has a molecular weight of greater than 150 000, and does not utilize alpha 1-4 linked glucose substrates larger than a disaccharide. Neutral alpha-glucosidase AB migrates more rapidly to the anode than alpha-glucosidase C when agarose, Cellogel, acrylamide or starch are used as support media. Both isozymes are equally inhibited by Zn2+.

References

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Citations

Nov 25, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·L PoenaruJ C Dreyfus
Nov 15, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·K PfisterB Steinmann
Dec 4, 2003·Pediatric Neurology·Judy R PipoKousaku Ohno
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·R HirschhornJ S Kasper
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·F MartiniukR Hirschhorn
Oct 28, 2008·Biochimica Et Biophysica Acta·Yoko Funakoshi, Tadashi Suzuki
Jun 1, 1984·Archives of Biochemistry and Biophysics·F MartiniukR Hirschhorn
May 15, 1991·Biochemical and Biophysical Research Communications·S Tzall, F Martiniuk
Apr 11, 2008·The Biochemical Journal·Aya IshizukaHisashi Narimatsu

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