Characterization of newly established clonal oviductal cell lines and differential hormonal regulation of gene expression

In Vitro Cellular & Developmental Biology. Animal
Tomohiro UmezuYasuhiro Tomooka

Abstract

Oviductal functions have been studied mainly in primary epithelial cell culture and organ culture. However, secretory cells and ciliated cells coexist in the epithelium, and the small size of the oviduct limits the sources of both epithelial and stromal cells. To circumvent the limits, we attempted to establish clonal cell lines from an oviduct of a p53-deficient mouse. An oviduct was enzymatically digested and cultured in medium containing 10% fetal calf serum supplemented with estradiol-17beta. Morphologically distinct clones (10 epithelial and 4 fibroblastic clones) were established, and all clones expressed estrogen receptor alpha and progesterone receptor. Expression of a mouse oviduct-specific glycoprotein gene as a marker of secretory cells was limited in one clone and was stimulated by estrogens and suppressed by progesterone. Expression of helix factor hepatocyte nuclear factor/forkhead homologue-4 gene as a marker of ciliated cells was limited in two clones and was suppressed by estrogens. The two genes were never coexpressed in any clones. The results strongly suggest that the oviductal epithelium consists of two functionally determined populations. To our knowledge, this is the first establishment of functional clon...Continue Reading

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Citations

Dec 25, 2009·In Vitro Cellular & Developmental Biology. Animal·Chiho TakahashiYasuhiro Tomooka
Feb 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Tomohiro UmezuYasuhiro Tomooka
Jun 30, 2011·Journal of Visualized Experiments : JoVE·Shelby M KingJoanna E Burdette
Sep 18, 2012·Biochemical and Biophysical Research Communications·Akihiko KomineTakafumi Uchida
Feb 27, 2007·Biochemical and Biophysical Research Communications·Akihiko KomineYasuhiro Tomooka
Aug 3, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yoko Inès NozawaPao-Tien Chuang
Nov 13, 2009·Biology of Reproduction·Hiromi YamanouchiYasuhiro Tomooka
Jul 1, 2011·Biology of Reproduction·Jin Gyoung JungJae Yong Han
Sep 12, 2009·Journal of Oncology·Alison M Karst, Ronny Drapkin

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