Characterization of NMB, GRP and their receptors (BRS3, NMBR and GRPR) in chickens

Journal of Molecular Endocrinology
Chunheng MoYajun Wang

Abstract

The two structurally and functionally related peptides, gastrin-releasing peptide (GRP) and neuromedin B (NMB) play critical roles in many physiological/pathological processes in mammals. However, the information regarding the expression and functionality of avian NMB, GRP and their receptors is limited. Here, we characterized cNMB, cGRP and their receptors (cNMBR, cGRPR and cBRS3) in chickens. Our results showed that: (1) cNMBR and cGRPR expressed in CHO cells could be potently activated by cNMB and cGRP, respectively, as monitored by cell-based luciferase reporter assays, indicating that cNMBR and cGRPR are cNMB- and cGRP-specific receptors; strikingly, BRS3 of chickens (/spotted gars), which is orthologous to mouse bombesin receptor subtype-3 (BRS3), could be potently activated by GRP and NMB, demonstrating that both peptides are the endogenous ligands for chicken (/spotted gar) BRS3; (2) quantitative real-time PCR (qPCR) revealed that cGRPR is widely expressed in chicken tissues with abundant expression in the ovary, pancreas, proventriculus, spinal cord and brain, whereas cNMB, cNMBR and cBRS3 are mainly expressed in the brain and testes; (3) interestingly, qPCR, Western blot and immunostaining revealed that cGRP is predom...Continue Reading

References

Sep 12, 1979·Biochemical and Biophysical Research Communications·T J McDonaldV Mutt
Nov 1, 1979·General and Comparative Endocrinology·S HarveyA Chadwick
Dec 1, 1975·European Journal of Pharmacology·G Linari, M B Linari
Sep 1, 1992·European Journal of Biochemistry·V GorbulevF Fahrenholz
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J F BatteyR I Feldman
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·V RettoriS M McCann
Jan 1, 1988·Annals of the New York Academy of Sciences·E Rozengurt
Jul 31, 1985·Biochemical and Biophysical Research Communications·N MinaminoH Matsuo
Nov 1, 1970·The Journal of Pharmacy and Pharmacology·V ErspamerM Inselvini
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E R SpindelJ F Habener
Feb 29, 1984·Biochemical and Biophysical Research Communications·N MinaminoH Matsuo
Jul 29, 1983·Biochemical and Biophysical Research Communications·N MinaminoH Matsuo
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·S R NagallaE R Spindel
Dec 12, 1994·European Journal of Pharmacology·E E LadenheimT H Moran
Feb 5, 2000·Neuropeptides·Z MeraliH Anisman
Aug 11, 2000·General and Comparative Endocrinology·T OhkuboK Nakashima
Nov 6, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Damien MatusiakRichard V Benya
May 21, 2005·The International Journal of Developmental Biology·Hiroko Ohki-HamazakiFumihiko Maekawa
Jun 23, 2005·Domestic Animal Endocrinology·Yong LiangXiquan Zhang
Aug 19, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Axel Meyer, Yves Van de Peer
Feb 8, 2006·Journal of Molecular Endocrinology·K J OliveiraC C Pazos-Moura
Aug 8, 2009·Science·Yan-Gang SunZhou-Feng Chen
Mar 18, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Tetsuya TachibanaMark A Cline
Aug 24, 2010·General and Comparative Endocrinology·Tetsuya TachibanaMark A Cline
Sep 3, 2010·American Journal of Physiology. Endocrinology and Metabolism·Joseph M MetzgerMarc L Reitman

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Citations

Apr 11, 2018·Journal of Molecular Endocrinology·Zhiyu MaJinlong Zhang
Feb 7, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yan ZhangOlivier Civelli
May 18, 2021·Circulation Research·Hirofumi WatanabeMaria Luisa S Sequeira-Lopez

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