Characterization of NO binding to human cystathionine beta-synthase: possible implications of the effects of CO and NO binding to the human enzyme

PMID: 11744062Dec 18, 2001Paper

Characterization of NO binding to human cystathionine beta-synthase: possible implications of the effects of CO and NO binding to the human enzyme

Journal of Inorganic Biochemistry

Shinichi Taoka, Ruma Banerjee

Abstract

Homocysteine is a key junction metabolite that can be converted to cystathionine in a reaction catalyzed by the heme and pyridoxal phosphate-dependent cystathionine beta-synthase. The heme has unusual spectroscopic properties and the axial ligands have been assigned as histidine and cysteine, respectively. Its role in the protein is not obvious from the chemistry of the beta-replacement reaction that is catalyzed. We have characterized the binding of the gaseous signaling molecule, NO, to cystathionine beta-synthase and examined its effect on the reactions catalyzed by the truncated dimeric form of the enzyme, W409X, which is a natural variant. Binding of NO appears to result in the formation of a five-coordinate ferrous nitrosyl species in which both endogenous ligands have been lost. This is in contrast to CO binding which is reported to displace the thiolate ligand and form a six-coordinate species. NO binds to the full-length enzyme with a K(d) of 281+/-50 microM and to the truncated enzyme with a K(d) of 350+/-44 microM. Binding of NO to the full-length enzyme inhibits activity with a K(i) of 320+/-60 microM. These studies demonstrate that as with CO, perturbation of the heme environment by NO is communicated to the active...Continue Reading

References

Nitric oxide synthase is a cytochrome P-450 type hemoprotein

Jul 28, 1992·Biochemistry·K A White, M A Marletta

Complexes of .NO with nucleophiles as agents for the controlled biological release of nitric oxide. Vasorelaxant effects

Nov 1, 1991·Journal of Medicinal Chemistry·C M MaragosL K Keefer

Neural-tube defects and derangement of homocysteine metabolism.

Jan 17, 1991·The New England Journal of Medicine·R P Steegers-TheunissenT K Eskes

Molecular cloning and sequence analysis of cDNA coding for rat liver hemoprotein H-450

Dec 1, 1990·Journal of Biochemistry·S IshiharaT Omura

Purification and some properties of cytochrome c' from a strain of Achromobacter xylosoxidans.

Jun 1, 1986·Journal of Biochemistry·S ShidaraA Nakahara

Electronic spectra for nitrosyl(protoporphyrin IX dimethyl ester)iron(II) and its complexes with nitrogenous bases as model systems for nitrosylhemoproteins

Feb 15, 1984·Archives of Biochemistry and Biophysics·T Yoshimura, T Ozaki

Carbon monoxide: a putative neural messenger

Jan 15, 1993·Science·A VermaS H Snyder

Homocysteine metabolism in pregnancies complicated by neural-tube defects

Jan 21, 1995·Lancet·J L MillsJ M Scott

Human cystathionine beta-synthase cDNA: sequence, alternative splicing and expression in cultured cells

Oct 1, 1993·Human Molecular Genetics·J P KrausV Kozich

Soluble guanylate cyclase from bovine lung: activation with nitric oxide and carbon monoxide and spectral characterization of the ferrous and ferric states

May 10, 1994·Biochemistry·J R Stone, M A Marletta

Spectral and kinetic studies on the activation of soluble guanylate cyclase by nitric oxide

Jan 30, 1996·Biochemistry·J R Stone, M A Marletta

Binding of nitric oxide and carbon monoxide to soluble guanylate cyclase as observed with Resonance raman spectroscopy

Feb 6, 1996·Biochemistry·G DeinumM A Marletta

Trans effects in nitric oxide binding to myoglobin cavity mutant H93G

Apr 16, 1996·Biochemistry·S M DecaturS G Boxer

"NONOates" (1-substituted diazen-1-ium-1,2-diolates) as nitric oxide donors: convenient nitric oxide dosage forms

Jan 1, 1996·Methods in Enzymology·L K KeeferD A Wink

A novel heme protein that acts as a carbon monoxide-dependent transcriptional activator in Rhodospirillum rubrum

Nov 21, 1996·Biochemical and Biophysical Research Communications·S AonoM Okada

The heme oxygenase system: a regulator of second messenger gases

Jan 1, 1997·Annual Review of Pharmacology and Toxicology·M D Maines

The homodimeric form of glycine N-methyltransferase acts as a polycyclic aromatic hydrocarbon-binding receptor

Aug 12, 1997·Biochemistry·R BhatE Bresnick

Effects of GTP on bound nitric oxide of soluble guanylate cyclase probed by resonance Raman spectroscopy

Aug 19, 1997·Biochemistry·T TomitaT Kitagawa

Analysis of CBS alleles in Czech and Slovak patients with homocystinuria: report on three novel mutations E176K, W409X and 1223 + 37 del99

Jul 1, 1997·Journal of Inherited Metabolic Disease·V KozichD Elleder

CooA, a CO-sensing transcription factor from Rhodospirillum rubrum, is a CO-binding heme protein

Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·D ShelverG P Roberts

Homocysteine and cardiovascular disease

Mar 24, 1998·Annual Review of Medicine·H RefsumS E Vollset

Correction of disease-causing CBS mutations in yeast

May 20, 1998·Nature Genetics·X Shan, W D Kruger

Trypsin cleavage of human cystathionine beta-synthase into an evolutionarily conserved active core: structural and functional consequences

Jul 24, 1998·Archives of Biochemistry and Biophysics·V KeryJ P Kraus

Structural changes in the heme proximal pocket induced by nitric oxide binding to soluble guanylate cyclase

Sep 9, 1998·Biochemistry·Y ZhaoM A Marletta

Evidence for heme-mediated redox regulation of human cystathionine beta-synthase activity.

Sep 17, 1998·The Journal of Biological Chemistry·S TaokaR Banerjee

Redox-controlled ligand exchange of the heme in the CO-sensing transcriptional activator CooA.

Sep 25, 1998·The Journal of Biological Chemistry·S AonoH Nakajima

Folate, vitamin B12, and serum total homocysteine levels in confirmed Alzheimer disease

Nov 21, 1998·Archives of Neurology·R ClarkeP M Ueland

Observation of a Fréedericksz transition in superfluid 3He-A

Oct 6, 1986·Physical Review Letters·J R HookH E Hall

Identification of two important heme site residues (cysteine 75 and histidine 77) in CooA, the CO-sensing transcription factor of Rhodospirillum rubrum

Mar 3, 1999·Biochemistry·D ShelverJ N Burstyn

Characterization of the heme and pyridoxal phosphate cofactors of human cystathionine beta-synthase reveals nonequivalent active sites

Mar 3, 1999·Biochemistry·S TaokaR Banerjee

EPR characterization of axial bond in metal center of native and cobalt-substituted guanylate cyclase.

Mar 13, 1999·The Journal of Biological Chemistry·R MakinoH Hori

The localization and interactions of huntingtin

Aug 6, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A L Jones

Assignment of enzymatic functions to specific regions of the PLP-dependent heme protein cystathionine beta-synthase

Oct 21, 1999·Biochemistry·S TaokaR Banerjee

Electronic absorption, EPR, and resonance raman spectroscopy of CooA, a CO-sensing transcription activator from R. rubrum, reveals a five-coordinate NO-heme

Jan 13, 2000·Biochemistry·M F ReynoldsT G Spiro

The mechanism of heme oxygenase

Apr 1, 2000·Current Opinion in Chemical Biology·P R Montellano

Yeast cystathionine beta-synthase is a pyridoxal phosphate enzyme but, unlike the human enzyme, is not a heme protein

Apr 15, 2000·The Journal of Biological Chemistry·K H JheeE W Miles

Characterization of the heme in human cystathionine beta-synthase by X-ray absorption and electron paramagnetic resonance spectroscopies

Aug 24, 2000·Biochemistry·S OjhaR Banerjee

Domain architecture of the heme-independent yeast cystathionine beta-synthase provides insights into mechanisms of catalysis and regulation

Aug 24, 2000·Biochemistry·K H JheeE W Miles

Inhibition of soluble guanylate cyclase by ODQ

Sep 9, 2000·Biochemistry·Y ZhaoM A Marletta

Characterization of variants altered at the N-terminal proline, a novel heme-axial ligand in CooA, the CO-sensing transcriptional activator

Sep 29, 2000·The Journal of Biological Chemistry·M V ThorsteinssonG P Roberts

Structure of the CO sensing transcription activator CooA

Oct 4, 2000·Nature Structural Biology·W N LanzilottaT L Poulos

Functional properties of the active core of human cystathionine beta-synthase crystals

Oct 24, 2000·The Journal of Biological Chemistry·S BrunoA Mozzarelli

Resonance Raman characterization of the heme cofactor in cystathionine beta-synthase. Identification of the Fe-S(Cys) vibration in the six-coordinate low-spin heme

Jan 10, 2001·Biochemistry·E L GreenT M Loehr

Pyridoxal phosphate binding sites are similar in human heme-dependent and yeast heme-independent cystathionine beta-synthases. Evidence from 31P NMR and pulsed EPR spectroscopy that heme and PLP cofactors are not proximal in the human enzyme

Mar 30, 2001·The Journal of Biological Chemistry·O KabilR Banerjee

Methionine metabolism in mammals

May 1, 1990·The Journal of Nutritional Biochemistry·J D Finkelstein

Evolutionary constraint on Otx2 neuroectoderm enhancers-deep conservation from skate to mouse and unique divergence in teleost

Dec 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Daisuke KurokawaShinichi Aizawa

Adducts between dansylated poly(propylene amine) dendrimers and anthracene clips mediated by Zn(II) ions: highly efficient photoinduced energy transfer.

Mar 14, 2009·Chemistry : a European Journal·Barbara BranchiFritz Vögtle

❮ Previous

Next ❯

Citations

Carbon monoxide: impact on remethylation/transsulfuration metabolism and its pathophysiologic implications.

Feb 15, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Takako HishikiMakoto Suematsu

Immunolocation of cystationine β-synthase in cerebral pontile nuclei in humans

Oct 17, 2013·Bulletin of Experimental Biology and Medicine·A E Kotsyuba, V M Chertok

The physiological role of hydrogen sulfide and beyond

Feb 5, 2014·Nitric Oxide : Biology and Chemistry·Hideo Kimura

Mercuric chloride-induced spin or ligation state changes in ferric or ferrous human cystathionine beta-synthase inhibit enzyme activity

Dec 18, 2001·Journal of Inorganic Biochemistry·S TaokaR Banerjee

Reversible heme-dependent regulation of human cystathionine β-synthase by a flavoprotein oxidoreductase.

Aug 31, 2011·Biochemistry·Omer KabilRuma Banerjee

Effect of the disease-causing R266K mutation on the heme and PLP environments of human cystathionine β-synthase.

Jun 29, 2012·Biochemistry·Aaron T SmithJudith N Burstyn

Comparative study of enzyme activity and heme reactivity in Drosophila melanogaster and Homo sapiens cystathionine β-synthases.

Sep 26, 2012·Biochemistry·Yang SuJudith N Burstyn

The transcription regulator RcoM-2 from Burkholderia xenovorans is a cysteine-ligated hemoprotein that undergoes a redox-mediated ligand switch.

Aug 5, 2008·Biochemistry·Katherine A MarvinJudith N Burstyn

Molecular regulation of tumor angiogenesis and perfusion via redox signaling.

Apr 21, 2009·Chemical Reviews·Thomas W MillerDavid D Roberts

Cobalt cystathionine β-synthase: a cobalt-substituted heme protein with a unique thiolate ligation motif.

Apr 13, 2011·Inorganic Chemistry·Aaron T SmithJudith N Burstyn

Quantitative vibrational dynamics of iron in nitrosyl porphyrins

Apr 1, 2004·Journal of the American Chemical Society·Bogdan M LeuJ Timothy Sage

Heme regulation of human cystathionine beta-synthase activity: insights from fluorescence and Raman spectroscopy.

Sep 3, 2009·Journal of the American Chemical Society·Colin L WeeksThomas G Spiro

Investigations of low-frequency vibrational dynamics and ligand binding kinetics of cystathionine beta-synthase.

Feb 17, 2010·The Journal of Physical Chemistry. B·Venugopal KarunakaranPaul M Champion

Properties of an unusual heme cofactor in PLP-dependent cystathionine beta-synthase

May 31, 2007·Natural Product Reports·Sangita SinghRuma Banerjee

H2S mediates O2 sensing in the carotid body.

Jun 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ying-Jie PengNanduri R Prabhakar

Hypoxic regulation of the cerebral microcirculation is mediated by a carbon monoxide-sensitive hydrogen sulfide pathway.

Jan 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Takayuki MorikawaMakoto Suematsu

Allosteric communication between the pyridoxal 5'-phosphate (PLP) and heme sites in the H2S generator human cystathionine β-synthase.

Sep 15, 2012·The Journal of Biological Chemistry·Pramod Kumar YadavRuma Banerjee

Detection of reaction intermediates during human cystathionine β-synthase-monitored turnover and H2S production.

Nov 6, 2012·The Journal of Biological Chemistry·Pramod Kumar Yadav, Ruma Banerjee

Dynamics of carbon monoxide binding to cystathionine beta-synthase.

Mar 1, 2006·The Journal of Biological Chemistry·Mrinalini PuranikThomas G Spiro

Redox biochemistry of hydrogen sulfide.

May 8, 2010·The Journal of Biological Chemistry·Omer Kabil, Ruma Banerjee

Cadmium exposure alters metabolomics of sulfur-containing amino acids in rat testes

May 14, 2005·Antioxidants & Redox Signaling·Yasoo SugiuraMakoto Suematsu

Interactions of multiple gas-transducing systems: hallmarks and uncertainties of CO, NO, and H2S gas biology.

Nov 27, 2009·Antioxidants & Redox Signaling·Mayumi KajimuraMakoto Suematsu

Hydrogen sulfide: neurophysiology and neuropathology

Sep 4, 2010·Antioxidants & Redox Signaling·Li-Fang HuJin-Song Bian

Neuroinflammation in the aging down syndrome brain; lessons from Alzheimer's disease.

Mar 29, 2012·Current Gerontology and Geriatrics Research·Donna M Wilcock

Down's syndrome, neuroinflammation, and Alzheimer neuropathogenesis

Jul 20, 2013·Journal of Neuroinflammation·Donna M Wilcock, W Sue T Griffin

A novel mechanism of formaldehyde neurotoxicity: inhibition of hydrogen sulfide generation by promoting overproduction of nitric oxide

Jan 30, 2013·PloS One·Xiao-Qing TangBi Hu

Nitrite reductase activity and inhibition of H₂S biogenesis by human cystathionine ß-synthase

Jan 15, 2014·PloS One·Carmen GherasimRuma Banerjee

Carbon monoxide stimulates global protein methylation via its inhibitory action on cystathionine β-synthase

Feb 8, 2011·Journal of Clinical Biochemistry and Nutrition·Takehiro YamamotoMakoto Suematsu

Enzymology of H2S biogenesis, decay and signaling

Apr 23, 2013·Antioxidants & Redox Signaling·Omer Kabil, Ruma Banerjee

Modulation of cystathionine beta-synthase activity by the Arg-51 and Arg-224 mutations

Sep 9, 2008·Bioscience, Biotechnology, and Biochemistry·Shin-Ichi OzakiKazuya Sada

Carbon monoxide as a guardian against hepatobiliary dysfunction

Dec 14, 2005·Alcoholism, Clinical and Experimental Research·Makoto SuematsuNobuhito Goda

Protein kinase G-regulated production of H2S governs oxygen sensing.

Apr 23, 2015·Science Signaling·Guoxiang YuanNanduri R Prabhakar

S-glutathionylation enhances human cystathionine β-synthase activity under oxidative stress conditions

Jun 4, 2014·Antioxidants & Redox Signaling·Wei-Ning NiuRuma Banerjee

Modes of physiologic H2S signaling in the brain and peripheral tissues

Apr 2, 2014·Antioxidants & Redox Signaling·Bindu D Paul, Solomon H Snyder

Signaling molecules: hydrogen sulfide and polysulfide

May 8, 2014·Antioxidants & Redox Signaling·Hideo Kimura

Interactions of the gasotransmitters contribute to microvascular tone (dys)regulation in the preterm neonate

Mar 26, 2015·PloS One·Rebecca M DysonIan M R Wright

Physiological Importance of Hydrogen Sulfide: Emerging Potent Neuroprotector and Neuromodulator

Jul 15, 2016·Oxidative Medicine and Cellular Longevity·Sandesh PanthiNa Young Jeong

CO and NO bind to Fe(II) DiGeorge critical region 8 heme but do not restore primary microRNA processing activity.

Oct 22, 2016·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Judy P HinesJudith N Burstyn

Comfortably Numb and Back: Plasma Metabolomics Reveals Biochemical Adaptations in the Hibernating 13-Lined Ground Squirrel

Dec 20, 2016·Journal of Proteome Research·Angelo D'AlessandroKirk C Hansen

Beyond a Gasotransmitter: Hydrogen Sulfide and Polysulfide in Cardiovascular Health and Immune Response

Apr 12, 2017·Antioxidants & Redox Signaling·Shuai YuanChristopher G Kevil

Catalytic promiscuity and heme-dependent redox regulation of H2 S synthesis

Mar 11, 2017·Current Opinion in Chemical Biology·Ruma Banerjee

Cystathionine β-synthase and PGRMC1 as CO sensors

Oct 23, 2016·Free Radical Biology & Medicine·Yasuaki KabeMakoto Suematsu

Redox regulation and reaction mechanism of human cystathionine-beta-synthase: a PLP-dependent hemesensor protein

Dec 8, 2004·Archives of Biochemistry and Biophysics·Ruma Banerjee, Cheng-Gang Zou

Reduced Production of Hydrogen Sulfide and Sulfane Sulfur Due to Low Cystathionine β-Synthase Levels in Brain Astrocytes of Stroke-Prone Spontaneously Hypertensive Rats

Dec 3, 2016·Biological & Pharmaceutical Bulletin·Sachiko JumanTomohiro Miki

Hydrogen sulfide and polysulfides as biological mediators

Oct 11, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hideo Kimura

A Review of Hydrogen Sulfide Synthesis, Metabolism, and Measurement: Is Modulation of Hydrogen Sulfide a Novel Therapeutic for Cancer?

May 24, 2018·Antioxidants & Redox Signaling·Xu CaoJin-Song Bian

Ischemia/reperfusion reduces transcription factor Sp1-mediated cystathionine beta-synthase expression in the kidney.

Apr 16, 2010·The Journal of Biological Chemistry·Nan WuKarmin O

A novel enhancing mechanism for hydrogen sulfide-producing activity of cystathionine beta-synthase

Sep 6, 2002·The Journal of Biological Chemistry·Ko Eto, Hideo Kimura

Cystathionine beta-synthase as a carbon monoxide-sensitive regulator of bile excretion

Dec 17, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Tsunehiro ShintaniMakoto Suematsu

Spectroscopic and DNA-binding characterization of the isolated heme-bound basic helix-loop-helix-PAS-A domain of neuronal PAS protein 2 (NPAS2), a transcription activator protein associated with circadian rhythms

May 18, 2006·The FEBS Journal·Yuji MukaiyamaToru Shimizu

❮ Previous

Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.