Characterization of non-oxidative transaldolase and transketolase enzymes in the pentose phosphate pathway with regard to xylose utilization by recombinant Saccharomyces cerevisiae

Enzyme and Microbial Technology
Akinori MatsushikaShinichi Yano

Abstract

The activity of transaldolase and transketolase, key enzymes in the non-oxidative pentose phosphate pathway, is rate-limiting for xylose utilization in recombinant Saccharomyces cerevisiae. Overexpression of TAL1 and TKL1, the major transaldolase and transketolase genes, increases the flux from the pentose phosphate pathway into the glycolytic pathway. However, the functional roles of NQM1 and TKL2, the secondary transaldolase and transketolase genes, especially in xylose utilization, remain unclear. This study focused on characterization of NQM1 and TKL2, together with TAL1 and TKL1, regarding their roles in xylose utilization and fermentation. Knockout or overexpression of these four genes on the phenotype of xylose-utilizing S. cerevisiae strains was also examined. Transcriptional analysis indicated that the expression of TAL1, NQM1, and TKL1 was up-regulated in the presence of xylose. A significant decrease in both growth on xylose and xylose-fermenting ability in tal1Δ and tkl1Δ mutants confirmed that TAL1 and TKL1 are essential for xylose assimilation and fermentation. Gene disruption analysis using a tkl1Δ mutant revealed that TKL1 is also required for utilization of glucose. Growth on xylose and xylose-fermenting abilit...Continue Reading

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Citations

Mar 26, 2013·Biotechnology Advances·Soo Rin KimJin-Ho Seo
Jan 2, 2007·Journal of Biotechnology·Alexander KernAnton Glieder
Sep 2, 2018·Biotechnology Journal·Venkatesh Endalur Gopinarayanan, Nikhil U Nair
Oct 5, 2014·Molecular and Cellular Biochemistry·Luka AndrisicAna Cipak Gasparovic
Jun 3, 2021·Biomolecules·Laura-Katharina BertelsJürgen J Heinisch

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