Characterization of nontypeable Actinobacillus pleuropneumoniae isolates.

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Ho ToChihiro Sasakawa

Abstract

Two Actinobacillus pleuropneumoniae isolates from clinical cases of porcine pleuropneumonia in Japan were positive in the capsular serovar 15-specific PCR assay, but nontypeable (NT) in the agar gel precipitation (AGP) test. Nucleotide sequence analysis of gene clusters involved in the biosynthesis of capsular polysaccharide (CPS) and lipopolysaccharide O-polysaccharide (O-PS) revealed that both clusters contained transposable element ISApl1 of A. pleuropneumoniae belonging to the IS30 family. Immunoblot analysis revealed that these 2 isolates could not produce O-PS. We conclude that the ISApl1 of A. pleuropneumoniae can interfere in the biosynthesis of both CPS and O-PS.

References

Apr 14, 1999·Molecular Microbiology·C Whitfield, I S Roberts
Nov 16, 2001·Animal Health Research Reviews·J D DubreuilM Gottschalk
Dec 4, 2001·Veterinary Microbiology·P J BlackallJ Frey
Oct 6, 2007·The Journal of Veterinary Medical Science·Tomohiro KoyamaShinya Nagai
Aug 31, 2010·Journal of Bacteriology·Zhuofei XuRui Zhou
Nov 13, 2014·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Hiroya Ito, Atsuko Matsumoto
Dec 17, 2014·The Journal of Veterinary Medical Science·Hiroya Ito, Masuo Sueyoshi
Oct 4, 2016·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Hiroya ItoMasahiro Kusumoto

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Datasets Mentioned

BETA
LC507611

Methods Mentioned

BETA
PCR
phosphotransferase
electrophoresis

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