Characterization of novel cell lines derived from a MYC-driven murine model of lethal metastatic adenocarcinoma of the prostate

The Prostate
Mark C MarkowskiAngelo M De Marzo

Abstract

Loss or mutation of PTEN alleles at 10q23 in combination with 8q24 amplification (encompassing MYC) are common findings in aggressive, human prostate cancer. Our group recently developed a transgenic murine model of prostate cancer involving prostate-specific Pten deletion and forced expression of MYC under the control of the Hoxb13 promoter. MYC overexpression cooperated with Pten loss to recapitulate lethal, human prostate cancer. We now report on the generation of two mouse prostate cancer cell lines, BMPC1 and BMPC2, derived from a lymph node, and liver metastasis, respectively. Both cell lines demonstrate a phenotype consistent with adenocarcinoma and grew under standard tissue culture conditions. Androgen receptor (AR) protein expression is minimal (BMPC1) or absent (BMPC2) consistent with AR loss observed in the BMPC mouse model of invasive adenocarcinoma. Growth in media containing charcoal-stripped serum resulted in an increase in AR mRNA in BMPC1 cells with no effect on protein expression, unless androgens were added, in which case AR protein was stabilized, and showed nuclear localization. AR expression in BMPC2 cells was not effected by growth media or treatment with androgens. Treatment with an anti-androgen/castra...Continue Reading

References

Dec 1, 1975·Acta Endocrinologica·J F NelsonC E Finch
Feb 5, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ryan P McMullinCharles J Bieberich
Jan 28, 2010·British Journal of Cancer·A H M ReidUNKNOWN Transatlantic Prostate Group
Jun 29, 2010·Cancer Cell·Barry S TaylorWilliam L Gerald
Feb 11, 2011·Nature·Michael F BergerLevi A Garraway
Sep 1, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Tamara L LotanAngelo M De Marzo
Jun 23, 2012·Nature·Catherine S GrassoScott A Tomlins
May 8, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ana M AparicioChristopher J Logothetis
May 23, 2013·British Journal of Cancer·J CuzickUNKNOWN Transatlantic Prostate Group
Jun 29, 2013·Seminars in Oncology·Randy Schrecengost, Karen E Knudsen
Apr 15, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Himisha BeltranMark A Rubin
Jul 16, 2014·Proceedings of the National Academy of Sciences of the United States of America·Haley HieronymusCharles L Sawyers
Oct 10, 2014·Molecular Cancer Research : MCR·Daniella Bianchi-FriasPeter S Nelson
Nov 7, 2015·Cell·UNKNOWN Cancer Genome Atlas Research Network
Jan 9, 2016·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Dec 31, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Matthias HoldhoffRavit Arav-Boger

❮ Previous
Next ❯

Citations

Oct 16, 2021·The Journal of Pathology·Janielle P MaynardKaren S Sfanos

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Virchows Archiv : an International Journal of Pathology
Justine Varinot, Eva Comperat
Virchows Archiv : an International Journal of Pathology
Valeria BarresiGiovanni Tuccari
Nihon rinsho. Japanese journal of clinical medicine
Yasuhide Miyoshi, Yoshinobu Kubota
© 2022 Meta ULC. All rights reserved