Characterization of novel elongated Parvulin isoforms that are ubiquitously expressed in human tissues and originate from alternative transcription initiation

BMC Molecular Biology
Jonathan W MuellerPeter Bayer

Abstract

The peptidyl prolyl cis/trans isomerase (PPIase) Parvulin (Par14/PIN4) is highly conserved in all metazoans and is assumed to play a role in cell cycle progression and chromatin remodeling. It is predominantly localized to the nucleus and binds to chromosomal DNA as well as bent oligonucleotides in vitro. In this study we confirm by RT-PCR the existence of a longer Parvulin isoform expressed in all tissues examined so far. This isoform contains a 5' extension including a 75 bp extended open reading frame with two coupled SNPs leading to amino acid substitutions Q16R and R18S. About 1% of all Parvulin mRNAs include the novel extension as quantified by real-time PCR. The human Parvulin promoter is TATA-less and situated in a CpG island typical for house keeping genes. Thus, different Parvulin mRNAs seem to arise by alternative transcription initiation. N-terminally extended Parvulin is protected from rapid proteinaseK degradation. In HeLa and HepG2 cell lysates two protein species of about 17 and 28 KDa are detected by an antibody against an epitope within the N-terminal extension. These two bands are also recognized by an antibody towards the PPIase domain of Parvulin. The longer Parvulin protein is encoded by the human genome b...Continue Reading

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Citations

May 10, 2011·Molecular Neurobiology·Melanie GerardVeerle Baekelandt
Nov 28, 2012·Biomolecular NMR Assignments·Yi-Jan LinChristian Lücke
Jul 2, 2014·Frontiers in Oncology·Tilman Polonio-VallonThomas G Hofmann
Jul 14, 2016·Journal of Medicinal Chemistry·Bryan M Dunyak, Jason E Gestwicki
Nov 9, 2011·Evolutionary Bioinformatics Online·Christoph LedererPeter Bayer
Oct 18, 2011·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·David DilworthChristopher J Nelson
Oct 19, 2017·Biological Chemistry·Anja MatenaPeter Bayer
May 6, 2015·The Journal of Biological Chemistry·Noelia Inés BurgardtMatthias Weiwad
Jan 1, 2008·Perspectives in Medicinal Chemistry·Jonathan W Mueller, Peter Bayer
Apr 19, 2013·Journal of Peptide Science : an Official Publication of the European Peptide Society·Imadeldin ElfakiPeter Bayer
Jul 16, 2011·Journal of Molecular Biology·Alexandra ThieleMatthias Weiwad

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Datasets Mentioned

BETA
AU098526
BI915994

Methods Mentioned

BETA
PCR
electrophoresis
co-immunoprecipitation
affinity purification
in vitro transcription

Software Mentioned

BLAST
Ensembl
BioEdit
tBLAST
Primer Express

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