Abstract
Brucellosis is a severe zoonotic disease worldwide. Detection and identification of Brucella species are essential to prevent or treat brucellosis in humans and animals. The outer membrane protein-31 (Omp31) is a major protein of Brucellae except for B. abortus, while the Omp31 antigenic epitopes have not been extensively characterized yet. A total of 22 monoclonal antibodies (mAbs) were produced against Omp31 of Brucella (B.) melitensis, of which 13 recognized five linear epitopes, 7 reacted with semi-conformational epitopes and 2 reacted with conformational epitopes, respectively. The mAb isotypes were 11 (50%) IgG2a, 5 (23%) IgG1 and 6 (27%) IgM. On the basis of epitope recognition and reactivity levels, 8 mAbs including 3 IgM and 5 IgG clones were considered as highly reactive and potentially diagnostic antibodies. Among these mAbs, 7A3 (IgG1), 5B1 (IgG2a), 2C1 (IgG2a) and 5B3 (IgG2a) reacted with differently conserved linear epitopes of B. melitensis, B. ovis, B. suis and B. canis strains, while 5H3 (IgG2a) highly reacted with a conformational epitope of Omp31 when tested with several immunoassays. These potent monoclonal antibodies can be used for identifying Omp31 antigens or detecting B. melitensis and other Brucella sp...Continue Reading
References
Dec 1, 1990·Infection and Immunity·A CloeckaertJ N Limet
Oct 1, 1995·Infection and Immunity·R A BowdenG Dubray
Sep 1, 1996·Infection and Immunity·N VizcaínoG Dubray
Oct 31, 1996·Nature·A K AbbasA Sher
Jun 24, 2000·Microbes and Infection·R A BowdenA Cloeckaert
Oct 13, 2001·Infection and Immunity·N VizcaínoL Fernández-Lago
Nov 5, 2002·Veterinary Microbiology·Shang DeqiuYin Jiming
Jan 13, 2004·Clinical and Diagnostic Laboratory Immunology·Juliana CassataroPablo C Baldi
Aug 26, 2004·Veterinary Microbiology·Silvia M EsteinRaúl A Bowden
Sep 24, 2005·Infection and Immunity·Juliana CassataroGuillermo H Giambartolomei
Nov 22, 2005·Infection and Immunity·Juliana CassataroGuillermo H Giambartolomei
Oct 4, 2006·Research in Veterinary Science·V K GuptaV S Vihan
Mar 8, 2007·Critical Reviews in Immunology·Cynthia L Baldwin, Radhika Goenka
Apr 20, 2007·Vaccine·Juliana CassataroFernando A Goldbaum
Nov 5, 2008·Infection and Immunity·Karina A PasquevichJuliana Cassataro
May 8, 2009·Journal of Clinical Microbiology·Maria Jesús Castaño, Javier Solera
Sep 15, 2009·Vaccine·Silvia M EsteinFernando A Goldbaum
Nov 4, 2009·Infection and Immunity·Marina A DurwardGary A Splitter
Apr 10, 2010·AIDS Research and Human Retroviruses·Kaustuv BanerjeeJohn P Moore
Dec 3, 2010·Emerging Infectious Diseases·Wen-Yi ZhangWu-Chun Cao
Jan 10, 2012·Journal of Translational Medicine·Maria Luisa ViscianoLuigi Buonaguro
May 2, 2012·Vaccine·V K GuptaG Splitter
Aug 24, 2012·PloS One·Ling ZhangChengyao Li
Jun 14, 2013·Clinical and Vaccine Immunology : CVI·Sapana TiwariKannusamy Sathyaseelan
Jul 31, 2013·PloS One·Yixin BianChengyao Li
Dec 29, 2013·Vaccine·Wenjing WangChengyao Li
Jan 1, 2014·Emerging Infectious Diseases·Shouyi ChenChengyao Li
Jun 6, 2014·PLoS Neglected Tropical Diseases·Kailash P PatraJoseph M Vinetz
Citations
May 7, 2020·Frontiers in Cellular and Infection Microbiology·Xin YangWenjing Wang
Feb 8, 2019·The Journal of Dairy Research·Bochao LiuTingting Li
Oct 23, 2019·Antimicrobial Resistance and Infection Control·Heng YangWenjing Wang
Jun 4, 2021·BMC Bioinformatics·Lindsay M W PielStephen N White
Sep 10, 2021·Frontiers in Microbiology·Bochao LiuChengyao Li