Characterization of novel Omp31 antigenic epitopes of Brucella melitensis by monoclonal antibodies

BMC Microbiology
Jinfeng LiChengyao Li

Abstract

Brucellosis is a severe zoonotic disease worldwide. Detection and identification of Brucella species are essential to prevent or treat brucellosis in humans and animals. The outer membrane protein-31 (Omp31) is a major protein of Brucellae except for B. abortus, while the Omp31 antigenic epitopes have not been extensively characterized yet. A total of 22 monoclonal antibodies (mAbs) were produced against Omp31 of Brucella (B.) melitensis, of which 13 recognized five linear epitopes, 7 reacted with semi-conformational epitopes and 2 reacted with conformational epitopes, respectively. The mAb isotypes were 11 (50%) IgG2a, 5 (23%) IgG1 and 6 (27%) IgM. On the basis of epitope recognition and reactivity levels, 8 mAbs including 3 IgM and 5 IgG clones were considered as highly reactive and potentially diagnostic antibodies. Among these mAbs, 7A3 (IgG1), 5B1 (IgG2a), 2C1 (IgG2a) and 5B3 (IgG2a) reacted with differently conserved linear epitopes of B. melitensis, B. ovis, B. suis and B. canis strains, while 5H3 (IgG2a) highly reacted with a conformational epitope of Omp31 when tested with several immunoassays. These potent monoclonal antibodies can be used for identifying Omp31 antigens or detecting B. melitensis and other Brucella sp...Continue Reading

References

Oct 31, 1996·Nature·A K AbbasA Sher
Nov 5, 2002·Veterinary Microbiology·Shang DeqiuYin Jiming
Jan 13, 2004·Clinical and Diagnostic Laboratory Immunology·Juliana CassataroPablo C Baldi
May 8, 2009·Journal of Clinical Microbiology·Maria Jesús Castaño, Javier Solera
Nov 4, 2009·Infection and Immunity·Marina A DurwardGary A Splitter
Dec 3, 2010·Emerging Infectious Diseases·Wen-Yi ZhangWu-Chun Cao
Jan 10, 2012·Journal of Translational Medicine·Maria Luisa ViscianoLuigi Buonaguro
Jan 1, 2014·Emerging Infectious Diseases·Shouyi ChenChengyao Li

❮ Previous
Next ❯

Datasets Mentioned

BETA
ADZ88512.1
ADZ67646.1
ACQ84164.1
AAL27294.1
AAL27292.1
AAL27290.1
AAL27287.1
AAL27296.1

Methods Mentioned

BETA
ELISA

Related Concepts

Related Feeds

Brucellosis

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

Brucellosis (ASM)

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.