Characterization of Novel Trichoderma asperellum Isolates to Select Effective Biocontrol Agents Against Tomato Fusarium Wilt

The Plant Pathology Journal
Mahmoud H El KomyYounes Y Molan

Abstract

The use of novel isolates of Trichoderma with efficient antagonistic capacity against Fusarium oxysporum f. sp. lycopersici (FOL) is a promising alternative strategy to pesticides for tomato wilt management. We evaluated the antagonistic activity of 30 isolates of T. asperellum against 4 different isolates of FOL. The production of extracellular cell wall degrading enzymes of the antagonistic isolates was also measured. The random amplified polymorphic DNA (RAPD) method was applied to assess the genetic variability among the T. asperellum isolates. All of the T. asperellum isolates significantly reduced the mycelial growth of FOL isolates but the amount of growth reduction varied significantly as well. There was a correlation between the antagonistic capacity of T. asperellum isolates towards FOL and their lytic enzyme production. Isolates showing high levels of chitinase and β-1,3-glucanase activities strongly inhibited the growth of FOL isolates. RAPD analysis showed a high level of genetic variation among T. asperellum isolates. The UPGMA dendrogram revealed that T. asperellum isolates could not be grouped by their anta- gonistic behavior or lytic enzymes production. Six isolates of T. asperellum were highly antagonistic tow...Continue Reading

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Citations

Jan 18, 2016·Plant Physiology and Biochemistry : PPB·Fuli ZhangChengwei Li
Nov 5, 2016·Molecular Plant-microbe Interactions : MPMI·M López-CoriaS Sánchez-Nieto
Sep 22, 2019·Journal of Applied Microbiology·Hong-Quan XianSong Yang
Apr 24, 2020·Journal of Applied Microbiology·Zainap Ab Easa HasanMohd Termizi Yusof
May 15, 2018·Frontiers in Microbiology·Magdalena FrącMałgorzata Jędryczka
Jun 22, 2020·Antonie van Leeuwenhoek·Heriberto Fortino Ramírez-CariñoRogelio Valadez-Blanco
Jun 15, 2019·Molecular Biology Reports·Nashwa M A SallamAhmed Sallam
Apr 14, 2021·Environmental Science and Pollution Research International·Sarmistha SarangiSayaji T Mehetre

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Methods Mentioned

BETA
PCR
electrophoresis
RAPD

Software Mentioned

pc
SAS
NTSYS

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