Characterization of OPA1 isoforms isolated from mouse tissues

Journal of Neurochemistry
Vasudheva Reddy AkepatiChristiane Alexander

Abstract

OPA1, a nuclear encoded mitochondrial protein causing autosomal dominant optic atrophy, is a key player in mitochondrial fusion and cristae morphology regulation. In the present study, we have compared the OPA1 transcription and translation products of different mouse tissues. Unlike in humans, we found only two exons (4b and 5b) to be involved in alternative splicing. The relative abundance of the resulting four different splice variants is tissue-dependent. Proteolytic cleavage by mitochondrial processing peptidase generates two long forms, isoforms 1 and 7, which lead to three short forms representing the end products after further proteolytic processing. In contrast, isoforms 5 and 8 are directly processed into their corresponding short forms. Short form 1 molecules form 184 kDa dimers, whereas all other isoforms contribute to 285 kDa complexes. Coiled-coil domains of the OPA1 protein specifically homo-associate and may be involved in the formation of these complexes. Furthermore, the region encoded by exon 5b inhibits the self-association of coiled-coil domain-I. Finally, our data pinpoint isoform 1 as the, by far, most abundant isoform in the nervous tissue. We postulate that manipulation of isoform 1 protein levels in re...Continue Reading

References

Nov 1, 1979·American Journal of Ophthalmology·P B JohnstonR C Tripathi
Dec 15, 1996·Experientia·P Luciano, V Géli
Oct 23, 1998·Journal of Medical Genetics·M VotrubaS S Bhattacharya
Apr 3, 1999·The Journal of Biological Chemistry·P M OkamotoR B Vallee
Dec 22, 1999·Biochemical and Biophysical Research Communications·A Ito
Mar 20, 2001·Nature Reviews. Neuroscience·B K DredgeR B Darnell
Jul 7, 2001·Human Molecular Genetics·C ToomesA J Churchill
Jul 17, 2001·Current Opinion in Cell Biology·D Danino, J E Hinshaw
Aug 22, 2001·Molecular Biology of the Cell·E SmirnovaA M van der Bliek
Jan 26, 2002·Human Genetics·C DelettreC P Hamel
Dec 25, 2002·The Journal of Cell Biology·Mariusz KarbowskiRichard J Youle
Dec 31, 2002·Biochemical and Biophysical Research Communications·Masaaki SatohHitoshi Endo
Feb 20, 2003·Protein Science : a Publication of the Protein Society·Livia OtteHartmut Oschkinat
Oct 28, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Stephanie BouePeer Bork
Feb 19, 2004·The Journal of Biological Chemistry·Lorena GriparicAlexander M van der Bliek
Feb 21, 2004·Current Topics in Developmental Biology·Hsiuchen Chen, David C Chan
Sep 10, 2004·Molecular Biology of the Cell·Yang-ja LeeRichard J Youle
Oct 29, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sara CipolatLuca Scorrano
Apr 16, 2005·Human Mutation·Marc FerréPascal Reynier
May 19, 2005·The Journal of Biological Chemistry·Hsiuchen ChenDavid C Chan
Jun 28, 2005·Nucleic Acids Research·Namshin KimHeebal Kim
Jan 18, 2006·Methods in Enzymology·Lorena Griparic, Alexander M van der Bliek
Jun 17, 2006·The EMBO Journal·Naotada IshiharaKatsuyoshi Mihara
Sep 28, 2006·The Journal of Biological Chemistry·Stéphane Duvezin-CaubetAndreas S Reichert
Feb 23, 2007·Brain : a Journal of Neurology·Marcel V AlaviBernd Wissinger
Jul 7, 2007·Molecular Biology of the Cell·Stéphane Duvezin-CaubetAndreas S Reichert

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Citations

Mar 22, 2013·PloS One·Jennifer J RahnSherine S L Chan
Jan 30, 2014·Science Signaling·Matthew NortonRobert A Screaton
Dec 20, 2012·Brain : a Journal of Neurology·Emmanuelle SarziGuy Lenaers
Dec 29, 2011·Frontiers in Bioscience (Elite Edition)·Indrajit ChowdhuryWinston E Thompson
Dec 30, 2009·The Journal of Cell Biology·Sarah EhsesThomas Langer
Jan 20, 2016·The Journal of Cell Biology·Anne KorwitzThomas Langer
Aug 4, 2009·Trends in Endocrinology and Metabolism : TEM·Michał Wasilewski, Luca Scorrano
Apr 25, 2009·The International Journal of Biochemistry & Cell Biology·Guy LenaersCécile Delettre
Apr 28, 2009·The International Journal of Biochemistry & Cell Biology·Luca Scorrano
May 19, 2009·Experimental Neurology·Takuma UoRichard S Morrison
Mar 14, 2009·Journal of Molecular and Cellular Cardiology·Jennifer Hom, Shey-Shing Sheu
Aug 26, 2009·The International Journal of Biochemistry & Cell Biology·Petr Jezek, Lydie Plecitá-Hlavatá
Oct 25, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R Anne StetlerJun Chen
Feb 23, 2012·The EMBO Journal·Elena I Rugarli, Thomas Langer
Dec 19, 2013·Journal of Cellular Physiology·Indrajit ChowdhuryKelwyn Thomas
Aug 12, 2009·Biochimica Et Biophysica Acta·Paola Martinelli, Elena I Rugarli
Nov 10, 2015·Neurobiology of Disease·Sophia Von StockumElena Ziviani
Apr 6, 2016·Epilepsy Research·Laura Córdova-DávalosRosalinda Guevara-Guzmán
May 31, 2014·Biochimica Et Biophysica Acta·Deborah Naon, Luca Scorrano
May 15, 2012·Biochimica Et Biophysica Acta·Luiza K Sanjuán Szklarz, Luca Scorrano
Aug 21, 2012·Biochimica Et Biophysica Acta·Pascale Belenguer, Luca Pellegrini
Aug 14, 2012·Biochimica Et Biophysica Acta·Mafalda Escobar-Henriques, Fabian Anton
Mar 30, 2010·Biochimica Et Biophysica Acta·Jennifer HomShey-Shing Sheu
Jun 27, 2015·Frontiers in Aging Neuroscience·Antonio Zorzano, Marc Claret
Mar 18, 2015·Biochimica Et Biophysica Acta·Luis Bonet-PonceMaría F Galindo
Oct 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Aditi SoodLuca Pellegrini
Nov 30, 2010·Progress in Retinal and Eye Research·Patrick Yu-Wai-ManPatrick F Chinnery
Jan 9, 2015·Antioxidants & Redox Signaling·Antonio ZorzanoJuan Pablo Muñoz
Apr 17, 2014·Trends in Neurosciences·Leslie A ScarffeTed M Dawson
Aug 16, 2016·Biochemical and Biophysical Research Communications·Marta Cherubini, Silvia Ginés
Apr 6, 2017·Journal of Cellular and Molecular Medicine·Mariame Selma KaneArnaud Chevrollier
Sep 25, 2017·Translational Stroke Research·Nina KlimovaTibor Kristian
Dec 18, 2013·Molecular and Cellular Biology·Sadhana A SamantMahesh P Gupta
Aug 6, 2016·PloS One·Haitham E El-SikhryJohn M Seubert
Feb 5, 2019·International Journal of Cancer. Journal International Du Cancer·Marcel V Alavi
Jul 9, 2009·Physiological Reviews·Marc LiesaAntonio Zorzano
Feb 23, 2020·Annual Review of Biochemistry·Soni DeshwalThomas Langer
Nov 5, 2013·Biological Chemistry·Begoña Ugarte-Uribe, Ana J García-Sáez
Sep 24, 2015·American Journal of Physiology. Endocrinology and Metabolism·Mei-Chen LoHorng-Mo Lee
Dec 17, 2020·Frontiers in Neuroscience·Daniel M MaloneyGwyneth Jane Farrar
Nov 26, 2020·Molecular Biology of the Cell·Ruohan WangDavid C Chan
Dec 20, 2020·Progress in Retinal and Eye Research·Guy LenaersBernd Wissinger

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