Characterization of optimized Na+ and Cl- liquid membranes for use with extracellular, self-referencing microelectrodes

Analytical and Bioanalytical Chemistry
Mark A MesserliPeter J S Smith

Abstract

Self-referencing with ion-selective microelectrodes (ISMs) is a useful approach for monitoring near-real-time ion flux near single cells and across epithelia. While ISMs for H+, Ca2+, and K+ have been optimized for use with self-referencing, ISMs for two other primary inorganic ions, Na+ and Cl-, have not. In this study, we have characterized ISMs based on three Na+ ionophores (I, VI, and X) and one Cl- ionophore to assess their suitability for use with self-referencing. ISMs constructed with Na+ ionophore VI have short response times (approximately 100 ms) but possess nearly an order of magnitude less selectivity for Na+ over K+ than ISMs constructed with Na+ ionophore X. The Na+ ionophore X mixture was enhanced to give it a shorter response time while not compromising its selectivity. A Cl(-)-selective microelectrode was constructed and characterized with superior anionic selectivity compared with previously reported Cl- ISMs used with self-referencing. This Cl(-)-selective microelectrode, however, has a relatively slow response time (approximately 3 s), thus requiring changes to the self-referencing protocol. Self-referencing with these ISMs will enable near-real-time ion flux measurements for Na+ and Cl-.

References

May 1, 1994·Renal Physiology and Biochemistry·J Pouysségur
Feb 1, 1995·The American Journal of Physiology·J Noël, J Pouysségur
Nov 18, 2004·The Plant Journal : for Cell and Molecular Biology·Mark A MesserliKenneth R Robinson
Jun 29, 2005·The Journal of Membrane Biology·S S GarberP J S Smith

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Citations

Oct 12, 2010·Plant & Cell Physiology·Roger R Lew
Aug 24, 2012·American Journal of Physiology. Cell Physiology·Aylin R RodanChou-Long Huang
Feb 25, 2014·Journal of Insect Physiology·Heath A MacMillan, Bryon N Hughson
Jan 25, 2011·Journal of Plant Physiology·Maheswari JayakannanZed Rengel
May 21, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Peter J S SmithMark A Messerli
Jul 7, 2015·Analytical Chemistry·Lukasz MendeckiAleksandar Radu
Jan 11, 2014·Journal of Insect Physiology·Evan K Pacey, Michael J O'Donnell
Dec 17, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Goce DimeskiAndrew St John
May 7, 2019·Current Medicinal Chemistry·Mark A Messerli, Anyesha Sarkar
Dec 13, 2019·Scientific Reports·Marino DiFrancoStephen C Cannon
Mar 13, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Heath A MacMillanJohannes Overgaard
Oct 1, 2011·The Journal of Experimental Biology·Wida Naikkhwah, Michael J O'Donnell
Mar 17, 2010·The Journal of Experimental Biology·J E HerrD S Fudge
Jul 4, 2013·The Journal of Experimental Biology·Austin Browne, Michael J O'Donnell
Jan 17, 2012·The Journal of Experimental Biology·Wida Naikkhwah, Michael J O'Donnell
Dec 20, 2014·The Journal of Experimental Biology·Heath A MacMillanBrent J Sinclair
May 20, 2020·ACS Applied Materials & Interfaces·Qi ZhangMark E Meyerhoff
Nov 7, 2018·ACS Sensors·Maral P S MousaviPhilippe Bühlmann

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